Abstract：
The virus family Phenuiviridae (order Hareavirales, comprising segmented negative-sense single stranded RNA viruses) has highly diverse members that are known to infect animals, plants, protozoans, and fungi. In this study, we identified a novel phenuivirus infecting a strain of the entomopathogenic fungus Cordyceps javanica isolated from a small brown plant hopper (Laodelphax striatellus), and this virus was tentatively named \"Cordyceps javanica negative-strand RNA virus 1\" (CjNRSV1). The CjNRSV1 genome consists of three negative-sense single stranded RNA segments (RNA1-3) with lengths of 7252, 2401, and 1117 nt, respectively. The 3\'- and 5\'-terminal regions of the RNA1, 2, and 3 segments have identical sequences, and the termini of the RNA segments are complementary to each other, reflecting a common characteristic of viruses in the order Hareavirales. RNA1 encodes a large protein (∼274 kDa) containing a conserved domain for the bunyavirus RNA-dependent RNA polymerase (RdRP) superfamily, with 57-80% identity to the RdRP encoded by phenuiviruses in the genus Laulavirus. RNA2 encodes a protein (∼79 kDa) showing sequence similarity (47-63% identity) to the movement protein (MP, a plant viral cell-to-cell movement protein)-like protein (MP-L) encoded by RNA2 of laulaviruses. RNA3 encodes a protein (∼28 kDa) with a conserved domain of the phenuivirid nucleocapsid protein superfamily. Phylogenetic analysis using the RdRPs of various phenuiviruses and other unclassified phenuiviruses showed CjNRSV1 to be grouped with established members of the genus Laulavirus. Our results suggest that CjNRSV1 is a novel fungus-infecting member of the genus Laulavirus in the family Phenuiviridae.
摘要：
病毒家族Phenuiviridae（OrderHareavirales，包含分段的负义单链RNA病毒）具有已知感染动物的高度多样性成员，植物,原生动物,和真菌。在这项研究中,我们发现了一种新的phenuivirus感染从一个小的棕色植物料斗（Laodelphoxstriatellus）中分离出的昆虫病原真菌虫草虫草，该病毒暂定名为“虫草负链RNA病毒1”(CjNRSV1)。CjNRSV1基因组由三个负义单链RNA片段（RNA1-3）组成，长度为7252、2401和1117nt，分别。RNA1、2和3个片段的3'-和5'-末端区域具有相同的序列，RNA片段的末端是互补的，反映了病毒的共同特征。RNA1编码一个包含布尼亚病毒RNA依赖性RNA聚合酶（RdRP）超家族保守域的大蛋白（~274kDa），与Laulavirus属中的phenuivirus编码的RdRP具有57-80％的同一性。RNA2编码一种与运动蛋白（MP，由laulavirus的RNA2编码的植物病毒细胞间运动蛋白）样蛋白（MP-L）。RNA3编码一种蛋白质（〜28kDa），该蛋白质具有苯类核壳蛋白超家族的保守域。使用各种表型病毒和其他未分类的表型病毒的RdRP进行的系统发育分析显示，CjNRSV1与Laulavirus属的已确定成员分组。我们的结果表明，CjNRSV1是Phenuiviridae家族中Laulavirus属的新型真菌感染成员。
