Abstract：
This research describes the eco-friendly green synthesis of silver nanoparticles employing Pongamia pinnata seed extracts loaded with nanogel formulations (AgNPs CUD NG) to improve the retention, accumulation, and the penetration of AgNPs into the epidermal layer of psoriasis. AgNPs were synthesized using the Box-Behnken design. Optimized AgNPs and AgNPs CUD NG were physico-chemically evaluated using UV-vis spectroscopy, SEM, FT-IR, PXRD, viscosity, spreadability, and retention studies. It was also functionally assessed using an imiquimod-induced rat model. The entrapment efficiency of AgNPs revealed ~ 79.35%. Physico-chemical parameters announced the formation of AgNPs via surface plasmon resonance and interaction between O-H, C = O, and amide I carbonyl group of protein extract and AgNO3. Optimized AgNPs showed spherical NPs ~ 116 nm with better physical stability and suitability for transdermal applications. AgNPs CUD NG revealed non-Newtonian, higher spreadability, and better extrudability, indicating its suitability for a transdermal route. AgNPs CUD NG enhanced the retention of AgNPs on the psoriatic skin compared to normal skin. Optimized formulations exhibit no irritation by the end of 72 h, indicating formulation safety. AgNPs CUD NG at a dose of 1 FTU showed significant recovery from psoriasis with a PASI score of ~ 0.8 compared to NG base and marketed formulations. Results indicated that seed extract-assisted AgNPs in association with CUD-based NG formulations could be a promising nanocarrier for psoriasis and other skin disorders.
摘要：
这项研究描述了采用载有纳米凝胶制剂（AgNPsCUDNG）的Pongamiapinnata种子提取物来改善保留率的银纳米颗粒的生态友好绿色合成，积累,和AgNPs渗透到银屑病的表皮层。使用Box-Behnken设计合成AgNP。优化的AgNPs和AgNPsCUDNG使用紫外-可见光谱进行物理化学评估，SEM,FT-IR,PXRD,粘度,铺展性，和保留研究。还使用咪喹莫特诱导的大鼠模型对其进行了功能评估。AgNPs的包封效率显示为~79.35%。物理化学参数通过表面等离子体共振和O-H之间的相互作用宣布了AgNPs的形成，C=O,以及蛋白质提取物的酰胺I羰基和AgNO3。优化的AgNPs显示出〜116nm的球形NPs,具有更好的物理稳定性和对透皮应用的适用性。AgNPsCUDNG显示非牛顿，更高的铺展性，和更好的可挤出性，表明其适用于透皮途径。与正常皮肤相比，AgNPsCUDNG增强了AgNPs在牛皮癣皮肤上的保留。优化的配方在72小时结束时没有刺激，表明配方安全。与NG基础和市售制剂相比，剂量为1FTU的AgNPsCUDNG显示出牛皮癣的显着恢复，PASI评分为〜0.8。结果表明,与基于CUD的NG制剂结合的种子提取物辅助的AgNP可能是用于牛皮癣和其他皮肤病的有希望的纳米载体。
