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Abstract：
Background: Osteoarthritis (OA) standing as the most prevalent form of arthritis, closely associates with heightened levels of reactive oxygen species, particularly hypochlorous acid (HOCl). Although there are numerous probes available for detecting HOCl in the OA region, probes with dual functions of diagnostic and therapeutic capabilities are still significantly lacking. While this type of probe can reduce the time gap between diagnosis and treatment, which is clinically needed. Methods: We developed a fluorescent probe (DHU-CBA1) toward HOCl with theranostics functions through the release of methylene blue (MB) and ibuprofen (IBP) in this work. DHU-CBA1 can detect HOCl with high specificity and sensitivity, releasing MB and IBP with an impressive efficiency of ≥ 95% in vitro. Results: DHU-CBA1 exhibits good biosafety, enabling in vivo imaging of endogenous HOCl, along with reducing arthritis scores, improving synovitis and cartilage damage, and maintaining catabolic balance while alleviating senescence in cartilage. Conclusions: This study proposes a novel approach to enhance osteoarthritis therapy by releasing IBP via a smart HOCl-enabled fluorescent probe.
摘要：
背景：骨关节炎（OA）作为关节炎的最常见形式，与活性氧水平升高密切相关，特别是次氯酸(HOCl)。尽管有许多探针可用于检测OA区域中的HOCl，具有诊断和治疗双重功能的探针仍然显着缺乏。虽然这种类型的探针可以减少诊断和治疗之间的时间间隔，这是临床上需要的。方法：在这项工作中，我们开发了一种针对HOCl的荧光探针（DHU-CBA1），该探针具有通过释放亚甲蓝（MB）和布洛芬（IBP）的治疗功能。DHU-CBA1检测HOCl具有较高的特异性和敏感性,体外释放MB和IBP的效率≥95％。结果:DHU-CBA1表现出良好的生物安全性,能够对内源性HOCl进行体内成像，同时降低关节炎评分，改善滑膜炎和软骨损伤，维持分解代谢平衡，同时减轻软骨衰老。结论：本研究提出了一种通过智能HOCl启用的荧光探针释放IBP来增强骨关节炎治疗的新方法。
