PDF(Pubmed)
Abstract：
Different rat strains are used in various animal models of pulmonary hypertension and right ventricular (RV) failure. No systematic assessment has been made to test differences in RV response to pressure overload between rat strains. We compared RV adaptation to pulmonary trunk banding (PTB) in Wistar (W), Sprague Dawley (SD), and Fischer344 (F) rats by hemodynamic profiling focusing on diastolic function. Age-matched male rat weanlings were randomized to sham surgery (W-sham, n = 5; SD-sham, n = 4; F-sham, n = 4) or PTB (W-PTB, n = 8; SD-PTB, n = 8; F-PTB, n = 8). RV function was evaluated after 5 weeks by echocardiography, cardiac MRI, and invasive pressure-volume measurements. PTB caused RV failure and increased RV systolic pressures four-fold in all three PTB groups compared with sham. W- and SD-PTB had a 2.4-fold increase in RV end-systolic volume index compared with sham, while F-PTB rats were less affected. Diastolic and right atrial impairment were evident by increased RV end-diastolic elastance, filling pressure, and E/e\' in PTB rats compared with sham, again F-PTB the least affected. In conclusions, PTB caused RV failure with signs of diastolic dysfunction. Despite a similar increase in RV systolic pressure, F-PTB rats showed less RV dilatation and a more preserved diastolic function compared with W- and SD-PTB.
摘要：
在肺动脉高压和右心室（RV）衰竭的各种动物模型中使用不同的大鼠品系。尚未进行系统评估以测试大鼠品系之间RV对压力超负荷的反应差异。我们比较了Wistar（W）中RV对肺干束带（PTB）的适应性，SpragueDawley(SD),和Fischer344（F）大鼠通过血流动力学分析关注舒张功能。年龄匹配的雄性大鼠断奶被随机分配到假手术(W-sham，n=5；SD-sham，n=4；F-sham，n=4)或PTB(W-PTB,n=8；SD-PTB，n=8；F-PTB，n=8)。5周后用超声心动图评价右心室功能,心脏MRI,和侵入式压力容积测量。与假手术相比，PTB在所有三个PTB组中导致RV衰竭并使RV收缩压增加四倍。与假手术相比，W-和SD-PTB的RV收缩末期容积指数增加2.4倍，而F-PTB大鼠受影响较小。右心房舒张和舒张末期弹性增加明显的舒张和右心房损害，灌装压力,与假手术相比，PTB大鼠的E/E'，再次F-PTB受影响最小。在结论中，PTB导致RV衰竭，伴有舒张功能障碍的迹象。尽管RV收缩压有类似的增加，与W-和SD-PTB相比，F-PTB大鼠显示更少的RV扩张和更保留的舒张功能。
