PDF(Pubmed)
Abstract：
High-altitude (HA) environment presents immense physiological adversities for humans that have been overcome by supplementing bio-active phytochemicals from functional foods that support and accelerate acclimatization under these extreme environmental conditions. Several agricultural interventions have been investigated to enhance the phytochemical content in vegetables however; these studies have been limited to low-altitude (LA) regions only. In view of an existing knowledge gap, current work is designed to compare the phytochemical compositions of HA and LA-grown Brassicaceae vegetables (cabbage, cauliflower, knol-khol, and radish) using organic treatments via farm yard manure (FYM) and Azotobacter. The open field study was conducted as a two-factorial randomized block design. The first factor was treatment (T1-FYM, T2-Azotobacter, T3-FYM + Azotobacter, and T4-control) while the second was locations (HA and LA). Among all these treatments, the application of treatment T3 in HA-grown cabbage showed the highest total phenolic content (TPC; 9.56 μg/mg), total flavonoids content (TFC; 14.48 μg/mg), and antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH; 85.97%) and ferric reducing antioxidant power (FRAP; 30.77 μg/mg) compared to LA grown samples. Reverse Phase high performance liquid chromatography (RP-HPLC) analysis showed that treatment T3 at HA led to significantly high kaempferol (0.92 μg/mg) and sulforaphane (8.94 μg/mg) contents in cabbage whereas, indole-3-carbinol (1.31 μg/mg) was higher in HA grown cauliflower. The present study provides scientific evidence for the enrichment of health-promoting phytochemical compounds in Brassicaceae vegetables grown with T3 treatment specifically at HA.
摘要：
高海拔（HA）环境为人类带来了巨大的生理逆境，这些逆境已通过从功能食品中补充生物活性植物化学物质来克服，这些功能食品在这些极端环境条件下支持和加速适应。已经研究了几种农业干预措施来提高蔬菜中的植物化学物质含量；这些研究仅限于低海拔（LA）地区。鉴于现有的知识差距，当前的工作旨在比较HA和LA种植的十字花科蔬菜的植物化学成分（卷心菜，花椰菜,knol-khol,和萝卜）通过农场粪便（FYM）和固氮菌进行有机处理。开放领域研究是以两因素随机区组设计进行的。第一个因素是治疗(T1-FYM，T2-固氮菌,T3-FYM+固氮菌,和T4对照），而第二个是位置（HA和LA）。在所有这些治疗中，处理T3在HA种植的白菜中显示出最高的总酚含量（TPC；9.56μg/mg），总黄酮含量（TFC；14.48μg/mg），与LA生长的样品相比，使用2,2-二苯基-1-吡啶酰肼（DPPH;85.97％）和三价铁还原抗氧化能力（FRAP;30.77μg/mg）的抗氧化潜力。反相高效液相色谱（RP-HPLC）分析表明，HA处理T3导致白菜中山奈酚（0.92μg/mg）和萝卜硫烷（8.94μg/mg）含量显着升高，而HA生长的花椰菜中吲哚-3-甲醇（1.31μg/mg）较高。本研究为T3处理特别是HA种植的十字花科蔬菜中促进健康的植物化学化合物的富集提供了科学证据。
