Abstract：
Perkinsus, a parasitic pathogen of marine bivalves, is widely distributed among various mollusks in numerous countries. However, the prevalence and diversity of Perkinsus species in the two economically important mussels, Mytilus coruscus and M. galloprovincialis, in China remain unknown. The presence of the Perkinsus species was identified in the two mussels sampled along the coast of the East China Sea and the Yellow Sea, using both the alternative Ray\'s fluid thioglycolate medium (ARFTM) and conventional polymerase chain reaction (PCR). The ARFTM test indicated the presence of Perkinsus-like hypnospores in the two mussels. The diameter of the hypnospores in M. coruscus was significantly smaller than that in M. galloprovincialis. The prevalence of Perkinsus in M. galloprovincialis and M. coruscus ranged from 0 to 37.5% and 0 to 25%, respectively. The mean intensity of Perkinsus in M. galloprovincialis and M. coruscus ranged from 0 to 5.14 and 0 to 4.92, respectively. The PCR assay showed that the prevalence of Perkinsus spp. in M. galloprovincialis and M. coruscus was 0 to 25.0% and 0 to 12.5%, respectively. The homology analysis of the newly obtained internal transcribed spacer (ITS) sequences of Perkinsus revealed the highest identity of 100% with P. beihaiensis. The phylogenetic analysis indicated that the Perkinsus isolates from the two mussels were clustered with P. beihaiensis. The results of the molecular biology indicated that only P. beihaiensis was detected in the two mussels. The highest prevalence of P. beihaiensis was observed in Liaoning province (Dalian, 20.83%), followed by Shandong province, Zhejiang province and Fujian province. Consequently, it is recommended that surveillance should be conducted in Dalian, where the prevalence and mean intensity of P. beihaiensis in M. galloprovincialis are the highest.
摘要：
珀金苏斯,海洋双壳类动物的寄生病原体，在许多国家的各种软体动物中广泛分布。然而,两种经济上重要的贻贝中Perkinsus物种的流行和多样性，Mytiluscoruscus和galloprovincialis，在中国仍然未知。在东海和黄海沿岸采样的两个贻贝中发现了Perkinsus物种的存在，使用替代Ray的巯基乙酸液体培养基（ARFTM）和常规聚合酶链反应（PCR）。ARFTM测试表明两个贻贝中存在类似于Perkinsus的催眠孢子。棘突中的催眠孢子直径明显小于galloprovincialis。Perkinsus在galloprovincialis和M.coruscus中的患病率为0至37.5％和0至25％，分别。Perkinsus的平均强度分别为0至5.14和0至4.92。PCR检测表明，Perkinsusspp的患病率。galloprovincialis和M.coruscus分别为0至25.0%和0至12.5%，分别。对新获得的Perkinsus的内部转录间隔区（ITS）序列进行的同源性分析显示，与北海假单胞菌具有100％的最高同一性。系统发育分析表明，从两个贻贝中分离出的Perkinsus菌株与北海假单胞菌成簇。分子生物学结果表明，在两个贻贝中只检测到了北海假单胞菌。在辽宁省（大连，20.83%），其次是山东省，浙江省和福建省。因此,建议在大连进行监测,其中柴胡的患病率和平均强度最高。
