Abstract：
In ornamental plants, artificial polyploidization has enabled the creation of new cultivars. Due to their high commercial value in the international flower market and their ornamental characteristics, such as the shape, size, color, and durability of their flower, orchids have received great attention in studies of artificial polyploidization. Here we described the protocol used for polyploid induction in Oncidium crispum, an epiphyte species native of southeastern Brazil, of great ornamental interest and widely sold in flower shops. The species stands out for having inflorescence with large flowers, brown in color with yellow spots. In addition, O. crispum has great potential for use in genetic improvement programs since the species is widely used in interspecific crosses. Closed capsules containing mature O. crispum seeds were subjected to running sterilized water for 10 min and then to a 1.5% sodium hypochlorite solution for 10 min. Small portions of seeds were introduced into 50 mL of water-soluble fertilizer with macro- and micronutrients (B>M) plus 0.7% agar. Explants originating from seeds previously in vitro germinated were submitted to 0.05% and 0.1% of colchicine for 4 days and 8 days. Flow cytometry and chromosome counts confirmed that the protocol successfully produced synthetic polyploid plants.
摘要：
在观赏植物中，人工多倍体化使新品种得以创造。由于其在国际花卉市场上的高商业价值和观赏特性，比如形状，尺寸,颜色,和它们花的耐久性，兰花在人工多倍体化研究中受到了极大的关注。在这里，我们描述了用于文心兰多倍体诱导的协议，巴西东南部的附生植物物种，具有极大的观赏兴趣，并在花店广泛销售。该物种因具有大花的花序而脱颖而出，棕色有黄色斑点。此外,O.crispum在遗传改良计划中具有巨大的潜力，因为该物种被广泛用于种间杂交。将含有成熟O.crispum种子的封闭胶囊在流动的无菌水中10分钟，然后在1.5％的次氯酸钠溶液中10分钟。将小部分的种子引入具有大量和微量营养素(B>M)加0.7%琼脂的50mL水溶性肥料中。将源自先前在体外发芽的种子的外植体给予0.05%和0.1%的秋水仙碱4天和8天。流式细胞术和染色体计数证实该方案成功地产生了合成多倍体植物。
