Abstract：
Over the years, our team has dedicated significant efforts to studying a unique natural dye-producing species, annatto (Bixa orellana L.). We have amassed knowledge and established foundations that support the applications of gene expression analysis in comprehending in vitro morphogenic regeneration processes, phase transition aspects, and bixin biosynthesis. Additionally, we have conducted gene editing associated with these processes. The advancements in this field are expected to enhance breeding practices and contribute to the overall improvement of this significant woody species. Here, we present a step-by-step protocol based on somatic embryogenesis and an optimized transformation protocol utilizing Agrobacterium tumefaciens.
摘要：
多年来,我们的团队致力于研究一种独特的天然染料生产物种，安纳托（比萨奥雷利亚娜L.）。我们已经积累了知识并建立了基础，支持基因表达分析在理解体外形态发生再生过程中的应用。相变方面，和Bixin生物合成。此外,我们已经进行了与这些过程相关的基因编辑。该领域的进步有望增强育种实践，并有助于这种重要的木本物种的整体改进。这里,我们提出了基于体细胞胚胎发生的分步方案和利用根癌农杆菌的优化转化方案。
