%0 Journal Article
%T Advances in Tissue Culture and Transformation Studies in Non-model Species: Bixa orellana L. (Bixaceae).
%A Otoni WC
%A Machado KLG
%A de Freitas Correia LN
%A Matos EM
%A Sousa ES
%A Silva LAS
%A Martins SB
%A Koehler AD
%A Neto VBP
%A Botelho MN
%A Faria DV
%A Cruz ACF
%A Mantovani NC
%A Rodrigues SM
%A Moreira VS
%A Soares VLF
%A Barrera-Rojas CH
%A Rocha DI
%A Xavier A
%A Viccini LF
%A Batista DS
%A Nogueira FTS
%A Costa MGC
%J Methods Mol Biol
%V 2827
%N 0
%D 2024
%M 38985274
暂无%R 10.1007/978-1-0716-3954-2_16
%X Over the years, our team has dedicated significant efforts to studying a unique natural dye-producing species, annatto (Bixa orellana L.). We have amassed knowledge and established foundations that support the applications of gene expression analysis in comprehending in vitro morphogenic regeneration processes, phase transition aspects, and bixin biosynthesis. Additionally, we have conducted gene editing associated with these processes. The advancements in this field are expected to enhance breeding practices and contribute to the overall improvement of this significant woody species. Here, we present a step-by-step protocol based on somatic embryogenesis and an optimized transformation protocol utilizing Agrobacterium tumefaciens.