Abstract：
An efficient procedure for in vitro propagation of Herreria salsaparrilha Martius was established from single-node explants (fourth and fifth nodes from apex to the base) derived from donor plants maintained under shading-house conditions. After surface sterilization, explants are inoculated in test tubes containing 15 mL of Murashige and Skoog (MS) medium without growth regulators. Cultures are maintained under 35 μmol m-2 s-1 irradiance, a 16/8-h light/dark light regime, at 26 ± 2 °C. The subcultures are carried out under the same conditions, adding 6-benzyladenine 1.0 mg/L and Phytagel® 2.8 g/L. Shoots are elongated and rooted by transferring individual shoots to half-strength MS medium without growth regulators. After 25-30 days, elongated rooted shoots are transferred to plastic pots containing 25-30 mL of sterile distilled water, covered with a transparent plastic bag, and kept under the same growth room conditions for 2 days. Plants are transferred to cups containing autoclaved and washed sand and kept in a shading house (50% light interception) for acclimatization. True-to-type adult plants were successfully recovered under ex vitro conditions.
摘要：
从单节点外植体（从顶点到基部的第四和第五节）建立了HerreriasalsaparrilhaMartius体外繁殖的有效程序，这些外植体来自在遮荫条件下维持的供体植物。表面灭菌后，将外植体接种在含有15mL不含生长调节剂的Murashige和Skoog(MS)培养基的试管中。培养物保持在35μmolm-2s-1辐照度下，16/8-h光/暗光状态，在26±2°C继代培养在相同的条件下进行，添加6-苄基腺嘌呤1.0mg/L和Phytagel®2.8g/L通过将单个枝条转移到没有生长调节剂的半强度MS培养基上，将枝条拉长并生根。25-30天后，将细长的生根芽转移到装有25-30mL无菌蒸馏水的塑料盆中，用一个透明的塑料袋覆盖，并在相同的生长室条件下保持2天。将植物转移到含有高压灭菌和洗涤过的沙子的杯子中，并保持在遮光室（50％的光截留）中以适应。在体外条件下成功回收了真正的成体植物。
