背景:由于植物具有巨大的治疗益处,因此可以极大地帮助植物发现和开发新的植物药,效率,成本效益,没有副作用,和更便宜的疗法。在这方面,橡皮,通常被称为橡木,用于治疗和预防各种人类疾病的民间医学,包括糖尿病.
目标:为此,本研究旨在评估Quercusbaloot的粗甲醇提取物和各种馏分的抗高脂血症和抗高血糖潜力,然后分析活性化合物。
方法:通过在四氧嘧啶诱导的糖尿病小鼠中口服150-300mg/kg的Q.baloot提取物14天来评估瑞士雄性白化病小鼠的降血糖和降血脂活性。
结果:结果显示,剂量为300mg/kg的粗甲醇提取物在第14天表现出血糖水平的显著降低(198.50±1.99mg/dl),并且与对照组相比,相同的处理在第14天显著增加了体重(31.26±0.27g)。此外,研究了高密度脂质(HDL)(30.33±0.33mg/dl)增加的生化参数,而低密度脂质(LDL)显示显着下降(105.66±0.26mg/dl)。此外,甘油三酯水平104.83±0.70mg/dl,总胆固醇185.50±0.76mg/dl显著下降。在血清生化分析肌酐和肝酶标志物中,血清谷氨酸丙酮酸转氨酶(32.00±0.36U/mg),血清谷氨酸草酰乙酸转氨酶(34.33±0.61U/mg),和碱性磷酸酶(157.00±0.73U/mg),与对照组相比,300mg/kg剂量的粗甲醇提取物显著减少。抗氧化酶如超氧化物歧化酶(4.57±0.011),在300mg/kg甲醇提取物的剂量下,过氧化物酶歧化酶(6.53±0.014,过氧化氢酶(8.38±0.014)显着增加。糖尿病心脏的组织病理学研究,肝脏,在甲醇提取物150和300mg/kg治疗组中,胰腺显示出受损组织的实质性恢复,支持Q.baloot种子的有效性。甲醇提取物的气相色谱-质谱分析确定了Q.baloot种子中的10种抗糖尿病活性化合物,验证抗高血糖活性。因此,150和300mg/kgQ.baloot剂量的甲醇粗提物显示出显着的抗高脂血症和抗高血糖活性,这验证了Q.baloot作为糖尿病治疗药物的民俗利用。
结论:结论:300mg/kg的Q.baloot甲醇提取物具有明显的降血糖和降血脂潜力,支持植物在治疗糖尿病及其并发症中的传统药用。纯化还需要进一步的研究,表征,和生物活性化合物的结构澄清。
BACKGROUND: The discovery and development of new phytomedicines can be greatly aided by plants because of their tremendous therapeutic benefits, efficiency, cost-effectiveness, lack of side effects, and cheaper therapies. In this regard, Quercus baloot, generally known as oak, is used in folkloric medicine for treating and preventing various human disorders, including diabetes.
OBJECTIVE: For this purpose, the present study aimed to evaluate crude methanolic extract and various fractions of Quercus baloot for antihyperlipidemic and antihyperglycemic potential followed by the analysis of active compounds.
METHODS: The hypoglycemic and hypolipidemic activity was evaluated in Swiss male Albino mice by administering an oral dose of 150-300 mg/kg of Q. baloot extracts in alloxan induced diabetic mice for 14 days.
RESULTS: The results revealed that crude methanolic extract at a dose of 300 mg/kg exhibited a significant reduction in the blood glucose level (198.50 ± 1.99 mg/dl) at day 14 and the same treatment significantly increased the body weight (31.26 ± 0.27g) at day 14 in comparison to the control group. Moreover, the biochemical parameters were investigated which presented an increase in high-density lipids (HDL) (30.33 ± 0.33 mg/dl), whereas Low-Density Lipids (LDL) showed a significant decrease (105.66 ± 0.26 mg/dl). Additionally, triglyceride levels 104.83 ± 0.70 mg/dl, and total cholesterol 185.50 ± 0.76 mg/dl are significantly decreased. In serum biochemical analysis creatinine and hepatic enzyme markers, like serum glutamate pyruvate transaminase (32.00 ± 0.36 U/mg), serum glutamate oxaloacetate transaminase (34.33 ± 0.61 U/mg), and alkaline phosphatase (157.00 ± 0.73 U/mg), were significantly reduced by the crude methanolic extract at a dose of 300 mg/kg as compared to the control group. The antioxidant enzymes like Superoxide dismutase (4.57 ± 0.011), peroxidases dismutase (6.53 ± 0.014, and catalase (8.38 ± 0.014) at a dosage of 300 mg/kg of methanolic extract exhibited a significant increase. The histopathological study of the diabetic heart, liver, and pancreas showed substantial restoration of damaged tissues in the methanolic extract 150 and 300 mg/kg treated group, which supports the effectiveness of Q. baloot seeds. The gas chromatography-mass spectrometry analysis of methanolic extract identified 10 antidiabetic active compounds in the Q. baloot seeds, validating the antihyperglycemic activity. Thus, methanolic crude extract at the doses 150 and 300 mg/kg of Q. baloot showed significant antihyperlipidemic and antihyperglycemic activities, which validate the folkloric utilization of Q. baloot as a remedy in diabetes.
CONCLUSIONS: In conclusion, the 300 mg/kg methanolic extract of Q. baloot has notable hypoglycemic and hypolipidemic potential, supporting the plant\'s traditional medicinal usage in the treatment of diabetes and its complications. Further studies are needed for the purification, characterization, and structural clarification of bioactive compounds.