Abstract：
Agaves are plants with multiple possibilities of use and are naturally tolerant to low water availability conditions and high temperatures. This makes them species of great interest in the context of the necessary substitution of crops due to climate change. Unfortunately, the overexploitation of wild specimens has endangered many species of the genus that have not been domesticated or cultivated intensively. In vitro mass culture and propagation techniques have emerged as a very efficient option to produce agave plants that can be used without damage to the natural populations. A protocol is presented here for the in vitro micropropagation of agaves in a two-stage process. In the first step, clusters of slightly differentiated shoots are generated from stem segments cultivated on a semisolid medium added with cytokinin. In a second step, these shoot clusters are cultured in temporary immersion bioreactors where they grow and complete their differentiation, and then the shoots are rooted and transferred to soil. This protocol has been successfully applied to several threatened species of the Agave genus.
摘要：
Agaves是具有多种使用可能性的植物，并且天然地耐受低水可用性条件和高温。这使得它们在气候变化导致的作物必要替代的背景下引起了极大的兴趣。不幸的是,野生标本的过度开发危及了许多尚未驯化或密集栽培的属物种。体外大规模培养和繁殖技术已成为生产龙舌兰植物的非常有效的选择，该龙舌兰植物可以在不损害自然种群的情况下使用。这里提出了一种在两阶段过程中进行龙舌兰体外微繁殖的方案。第一步,从在添加细胞分裂素的半固体培养基上培养的茎段产生略微分化的芽簇。第二步,这些芽簇在临时浸没生物反应器中培养，在那里它们生长并完成分化，然后将枝条生根并转移到土壤中。该方案已成功应用于龙舌兰属的几种受威胁物种。
