Abstract：
METHODS: Polar lipids, such as gangliosides and phospholipids, are fundamental structural components that play critical roles in the development and maturation of neurons in the brain. Recent evidence has demonstrated that dietary intakes of polar lipids in early life are associated with improved cognitive outcomes during infancy and adolescence. However, the specific mechanisms through which these lipids impact cognition remain unclear.
RESULTS: This study examines the direct physiological impact of polar lipid supplementation, in the form of buttermilk powder, on primary cortical neuron growth and maturation. The changes are measured with postsynaptic current response recordings, immunohistochemical examination of functional synapse localization and numbers, and the biochemical quantification of receptors responsible for neuronal synaptic neurotransmission. Chronic exposure to polar lipids increases primary mouse cortical neuron basal excitatory synapse response strength attributed to enhanced dendritic complexity and an altered expression of the excitatory α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit 2 (GluR2).
CONCLUSIONS: The present finding suggests that dietary polar lipids improve human cognition through an enhancement of neuronal maturation and/or function.
RESULTS: This study examines the direct physiological impact of polar lipid supplementation, in the form of buttermilk powder, on primary cortical neuron growth and maturation. The changes are measured with postsynaptic current response recordings, immunohistochemical examination of functional synapse localization and numbers, and the biochemical quantification of receptors responsible for neuronal synaptic neurotransmission. Chronic exposure to polar lipids increases primary mouse cortical neuron basal excitatory synapse response strength attributed to enhanced dendritic complexity and an altered expression of the excitatory α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit 2 (GluR2).
CONCLUSIONS: The present finding suggests that dietary polar lipids improve human cognition through an enhancement of neuronal maturation and/or function.
摘要：
方法：极性脂质，如神经节苷脂和磷脂，是在大脑中神经元的发育和成熟中起关键作用的基本结构成分。最近的证据表明，早期饮食中极性脂质的摄入与婴儿期和青春期认知结果的改善有关。然而,这些脂质影响认知的具体机制尚不清楚.
结果：本研究检查了极性脂质补充的直接生理影响，以酪乳粉的形式，对原代皮层神经元生长和成熟的影响。这些变化是用突触后电流反应记录来测量的，功能突触定位和数量的免疫组织化学检查，以及负责神经元突触神经传递的受体的生化定量。长期暴露于极性脂质可增加小鼠皮层神经元基础兴奋性突触反应强度，这归因于树突复杂性增强和兴奋性α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体亚基2（GluR2）的表达改变。
结论：本发现提示膳食极性脂质通过增强神经元成熟和/或功能来改善人类认知。
结果：本研究检查了极性脂质补充的直接生理影响，以酪乳粉的形式，对原代皮层神经元生长和成熟的影响。这些变化是用突触后电流反应记录来测量的，功能突触定位和数量的免疫组织化学检查，以及负责神经元突触神经传递的受体的生化定量。长期暴露于极性脂质可增加小鼠皮层神经元基础兴奋性突触反应强度，这归因于树突复杂性增强和兴奋性α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体亚基2（GluR2）的表达改变。
结论：本发现提示膳食极性脂质通过增强神经元成熟和/或功能来改善人类认知。
