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Abstract：
There is no precise information available on the entire workload of isolating a specific microorganism in a clinical microbiology laboratory, and the costs associated with it have not been specifically estimated. In this descriptive retrospective study conducted at the microbiology department of a general teaching hospital from January 2021 to December 2022, we assessed the workload associated with identifying Candida species in all types of clinical samples and patients. Costs were estimated from data obtained from the hospital\'s finance department and microbiology laboratory cost records. In 2 years, 1,008,231 samples were processed at our microbiology department, of which 8,775 had one or more Candida spp. isolates (9,683 total isolates). Overall, 5,151 samples with Candida spp. were identified from 2,383 inpatients. We isolated Candida spp. from 515.3 samples/100,000 population/year and from 92 samples/1,000 hospital admissions/year. By sample type, 90.8% were superficial, mainly mucosal. Only 9.1% Candida spp. were isolated from deep, usually sterile, samples, being mostly from ordinarily sterile fluids. Candida albicans was the main species (58.5%) identified, followed by C. parapsilosis complex, C. glabrata, C. tropicalis, and C. krusei. In admitted patients, the incidences of samples with Candida spp. isolates were 302.7 samples/100,000 population/year and 54 samples/1,000 admissions/year. The average cost of isolating and identifying Candida spp. was estimated at 25€ per culture-positive sample. To our knowledge, this is the first attempt to gage the workload and costs of Candida spp. isolation at a hospital microbiology department. These data can help assess the burden and significance of Candida isolation at other institutions and also help design measures for streamlining.
OBJECTIVE: We believe that this work is of interest because at present, there is no really accurate information available on the total workload involved in isolating a specific microorganism in a clinical microbiology laboratory. The costs related to this have also not been described. We have described the unrestricted workload of Candida spp. in all types of samples for all types of species and patients. We believe that this information would be necessary to collect and share this information as well as to collect it in a standardized way to know the current situation of Candida spp. workload in all clinical microbiology laboratories.
OBJECTIVE: We believe that this work is of interest because at present, there is no really accurate information available on the total workload involved in isolating a specific microorganism in a clinical microbiology laboratory. The costs related to this have also not been described. We have described the unrestricted workload of Candida spp. in all types of samples for all types of species and patients. We believe that this information would be necessary to collect and share this information as well as to collect it in a standardized way to know the current situation of Candida spp. workload in all clinical microbiology laboratories.
摘要：
在临床微生物学实验室中，没有关于分离特定微生物的全部工作量的准确信息，与之相关的成本尚未具体估算。在2021年1月至2022年12月在综合教学医院微生物学部门进行的这项描述性回顾性研究中，我们评估了与识别所有类型临床样本和患者中的念珠菌种类相关的工作量。根据从医院财务部门和微生物实验室成本记录获得的数据估算成本。在两年内,我们的微生物学部门处理了1,008,231个样本，其中8,775个有一个或多个念珠菌属。分离株（总计9,683株）。总的来说,5,151个带有念珠菌的样品。从2383名住院患者中确定。我们分离了念珠菌。从515.3个样本/100,000个人口/年和从92个样本/1,000个住院/年。按样品类型，90.8%是肤浅的，主要是粘膜。只有9.1%的念珠菌属。与深处隔绝，通常是无菌的，样品,主要来自通常无菌的液体。白色念珠菌是确定的主要物种(58.5%)，紧随其后的是近扁平梭菌复合体，C.光滑，C.热带，还有C.krusei.在入院的患者中，念珠菌属样品的发生率。分离株为302.7个样本/100,000个人口/年和54个样本/1,000个入院/年。分离和鉴定念珠菌的平均成本。估计每个培养阳性样本为25欧元。据我们所知,这是首次尝试测量念珠菌属的工作量和成本。在医院微生物科隔离.这些数据可以帮助评估其他机构念珠菌隔离的负担和重要性，也有助于设计精简措施。
目的：我们认为这项工作很有意义，因为目前，在临床微生物学实验室中，没有关于分离特定微生物所涉及的总工作量的准确信息。与此相关的费用也没有描述。我们已经描述了念珠菌属的无限制工作量。在所有类型的物种和患者的所有类型的样本中。我们认为，这些信息对于收集和共享这些信息以及以标准化的方式收集以了解念珠菌属的现状是必要的。所有临床微生物学实验室的工作量。
目的：我们认为这项工作很有意义，因为目前，在临床微生物学实验室中，没有关于分离特定微生物所涉及的总工作量的准确信息。与此相关的费用也没有描述。我们已经描述了念珠菌属的无限制工作量。在所有类型的物种和患者的所有类型的样本中。我们认为，这些信息对于收集和共享这些信息以及以标准化的方式收集以了解念珠菌属的现状是必要的。所有临床微生物学实验室的工作量。
