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Abstract：
This study aims to explore the link between retinal vein occlusion (RVO), a blinding ocular condition, and alterations in gut microbiota composition, to offer insights into the pathogenesis of RVO. Fecal samples from 25 RVO patients and 11 non-RVO individuals were analyzed using 16S rRNA sequencing and liquid chromatography-mass spectrometry (LC-MS). Significant differences in the abundance of gut microbial species were noted between RVO and non-RVO groups. At the phylum level, the RVO group showed an elevation in the ratio of Firmicutes to Bacteroidetes. At the genus level, the RVO group showed higher abundance in Escherichia_Shigella (P < 0.05) and less abundance in Parabacteroides (P < 0.01) than the non-RVO group. Functional predictions indicated reduced folate synthesis, biotin metabolism, and oxidative phosphorylation, with an increase in butyric acid metabolism in the RVO group. LC-MS analysis showed significant differences in purine metabolism, ABC transporters, and naphthalene degradation pathways, especially purine metabolism. Pearson correlation analysis revealed significant associations between bacterial genera and fecal metabolites. Enrichment analysis highlighted connections between specific metabolites and bacterial genera. The findings showed that the dysregulation of gut microbiota was observed in RVO patients, suggesting the gut microbiota as a potential therapeutic target. Modulating the gut microbiota could be a novel strategy for managing RVO and improving patient outcomes. Furthermore, the study findings suggest the involvement of gut microbial dysbiosis in RVO development, underscoring the significance of understanding its pathogenesis for effective treatment development.
OBJECTIVE: Retinal vein occlusion (RVO) is a blinding ocular condition, and understanding its pathogenesis is crucial for developing effective treatments. This study demonstrates significant differences in gut microbiota composition between RVO patients and non-RVO individuals, implicating the involvement of gut microbial dysbiosis in RVO development. Functional predictions and metabolic profiling provide insights into the underlying mechanisms, highlighting potential pathways for therapeutic intervention. These findings suggest that modulating the gut microbiota might be a promising strategy for managing RVO and improving patient outcomes.
OBJECTIVE: Retinal vein occlusion (RVO) is a blinding ocular condition, and understanding its pathogenesis is crucial for developing effective treatments. This study demonstrates significant differences in gut microbiota composition between RVO patients and non-RVO individuals, implicating the involvement of gut microbial dysbiosis in RVO development. Functional predictions and metabolic profiling provide insights into the underlying mechanisms, highlighting potential pathways for therapeutic intervention. These findings suggest that modulating the gut microbiota might be a promising strategy for managing RVO and improving patient outcomes.
摘要：
本研究旨在探讨视网膜静脉阻塞(RVO),失明的眼部状况，以及肠道微生物群组成的改变，提供对RVO发病机制的见解。使用16SrRNA测序和液相色谱-质谱(LC-MS)分析来自25名RVO患者和11名非RVO个体的粪便样品。在RVO和非RVO组之间注意到肠道微生物物种的丰度的显著差异。在门一级，RVO组显示厚壁菌与拟杆菌的比例升高。在属一级，与非RVO组相比，RVO组表现出更高的大肠杆菌_志贺氏菌丰度（P<0.05），而副杆菌属丰度较低（P<0.01）。功能预测表明叶酸合成减少，生物素代谢，和氧化磷酸化，随着RVO组丁酸代谢的增加。LC-MS分析显示嘌呤代谢存在显著差异,ABC运输商，和萘降解途径，尤其是嘌呤代谢。Pearson相关性分析显示细菌属和粪便代谢产物之间存在显著关联。富集分析强调了特定代谢物和细菌属之间的联系。研究结果表明，在RVO患者中观察到肠道菌群失调，提示肠道微生物群是潜在的治疗靶点。调节肠道微生物群可能是管理RVO和改善患者预后的新策略。此外，研究结果表明，肠道微生物菌群失调参与RVO的发育，强调了解其发病机制对有效治疗发展的重要性。
目的：视网膜静脉阻塞（RVO）是一种致盲眼病，了解其发病机制对于开发有效的治疗方法至关重要。这项研究表明，RVO患者和非RVO个体之间的肠道菌群组成存在显着差异。肠道微生物菌群失调参与RVO发育。功能预测和代谢分析提供了对潜在机制的见解，突出治疗干预的潜在途径。这些发现表明，调节肠道微生物群可能是管理RVO和改善患者预后的有希望的策略。
目的：视网膜静脉阻塞（RVO）是一种致盲眼病，了解其发病机制对于开发有效的治疗方法至关重要。这项研究表明，RVO患者和非RVO个体之间的肠道菌群组成存在显着差异。肠道微生物菌群失调参与RVO发育。功能预测和代谢分析提供了对潜在机制的见解，突出治疗干预的潜在途径。这些发现表明，调节肠道微生物群可能是管理RVO和改善患者预后的有希望的策略。
