Abstract：
Intermediate filaments (IFs) are key molecular factors of the cell and have been reported to play an important role in maintaining the structural integrity and functionality of the abomasum. This study was designed to determine the regional distribution, cellular localization and expression of several IFs, including CK8, CK18, CK19, vimentin, desmin, peripherin and nestin, as well as the connective tissue component laminin, in the bovine, ovine and caprine abomasa. Immunohistochemical analyses demonstrated varying levels of expression of CK8, CK18, CK19, vimentin, desmin, nestin, peripherin and laminin in the bovine, ovine and caprine abomasa. CK8 immunoreactions were particularly evident in the luminal and glandular epithelia of the glands found in the abomasal cardia, fundus and pylorus in all three species. In the bovine abomasum, CK18 immunoreactions were stronger in the parietal cells, compared to the chief cells. In the abomasum of all three species, the smooth muscle as well as the smooth muscle cells of the vascular media in the cardiac, fundic and pyloric regions showed strong immunoreactivity. In all three species, the cardiac, fundic and pyloric regions of the abomasum showed strong peripherin and nestin immunoreactions in the luminal and glandular epithelial cells, stromal and smooth muscle cells, nervous plexuses and blood vessels. The expression patterns of IFs and laminin in the ruminant abomasum suggest that these proteins play a structural role in the cytoskeleton and are effective in maintaining abomasal tissue integrity and stability.
摘要：
中间丝（IF）是细胞的关键分子因子，据报道在维持皱胃的结构完整性和功能中起重要作用。这项研究旨在确定区域分布，几种IFs的细胞定位和表达，包括CK8，CK18，CK19，波形蛋白，desmin,外周蛋白和巢蛋白，以及结缔组织成分层粘连蛋白，在牛身上，绵羊和山羊恶臭。免疫组织化学分析显示不同水平的CK8，CK18，CK19，波形蛋白，desmin,Nestin,牛的外周蛋白和层粘连蛋白，绵羊和山羊恶臭。CK8免疫反应在房底贲门中发现的腺体的腔和腺上皮中特别明显，这三个物种的眼底和幽门。在牛皱胃中，CK18免疫反应在壁细胞中更强，与主要细胞相比。在这三个物种的皱胃中，平滑肌以及心脏血管介质的平滑肌细胞，胃底和幽门区域显示出强的免疫反应性。在所有三个物种中，心脏,皱胃的胃底和幽门区域在腔和腺上皮细胞中显示出强烈的外周蛋白和巢蛋白免疫反应，基质和平滑肌细胞，神经丛和血管.反刍动物皱胃中IFs和层粘连蛋白的表达模式表明，这些蛋白质在细胞骨架中起结构作用，并有效维持皱胃组织的完整性和稳定性。
