PDF(Pubmed)
Abstract：
Antimicrobial photodynamic therapy (aPDT) has emerged as a highly promising strategy for non-antibiotic treatment of infections due to its unique advantages in efficient bactericidal action and reduction of drug resistance. The natural photosensitizing properties of curcumin (Cur) are widely acknowledged; however, its limited bioavailability has impeded its practical application. In this study, we developed a nanomaterial called Cur@ZIF-8@BA by encapsulating Cur within ZIF-8 and modifying the surface with boric acid (BA). The Cur@ZIF-8@BA exhibits pH-responsive properties and enhances bacterial binding, thereby effectively promoting photodynamic therapy. Moreover, its antibacterial activity against E. coli, Staphylococcus aureus and A. baumannii is significantly increased in the presence of light compared to a dark environment. The mechanism behind this may be that BA increases the affinity of Cur@ZIF-8@BA towards bacteria, and making released Zn2+ and BA from the nanomaterial increase bacterial cell membrane permeability. This facilitates efficient delivery of Cur into bacterial cells, resulting in generation of abundant reactive oxygen species (ROS) and subsequent bactericidal activity. In conclusion, our prepared Cur@ZIF-8@BA holds great promise as a photodynamically mediated antimicrobial strategy.
摘要：
抗微生物光动力疗法（aPDT）由于其在有效杀菌作用和减少耐药性方面的独特优势，已成为非抗生素治疗感染的非常有前途的策略。姜黄素（Cur）的天然光敏特性得到了广泛认可；然而，其有限的生物利用度阻碍了其实际应用。在这项研究中,我们通过将Cur封装在ZIF-8中并用硼酸（BA）修饰表面，开发了一种名为Cur@ZIF-8@BA的纳米材料。Cur@ZIF-8@BA具有pH响应特性并增强细菌结合，从而有效地促进光动力疗法。此外，它对大肠杆菌的抗菌活性，与黑暗环境相比,金黄色葡萄球菌和鲍曼不动杆菌在光存在下显著增加。这背后的机制可能是BA增加Cur@ZIF-8@BA对细菌的亲和力，并使从纳米材料中释放的Zn2和BA增加细菌细胞膜的通透性。这有助于将Cur有效地递送到细菌细胞中，产生丰富的活性氧（ROS）和随后的杀菌活性。总之,我们制备的Cur@ZIF-8@BA作为光动力学介导的抗菌策略具有很大的前景。
