Abstract：
Organisms display a remarkable diversity in their shapes. Although substantial progress has been made in unraveling the mechanisms that govern cell fate determination during development, the mechanisms by which fate-determined cells give rise to the final shapes of organisms remain largely unknown. This study describes in detail the process of the final shape formation of the tarsus, which is near the distal tip of the adult leg, during the pupal stage in Drosophila melanogaster. Days-long live imaging revealed unexpectedly complicated cellular dynamics. The epithelial cells transiently form the intriguing structure, which we named the Parthenon-like structure. The basal surface of the epithelial cells and localization of the basement membrane protein initially show a mesh-like structure and rapidly shrink into the membranous structure during the formation and disappearance of the Parthenon-like structure. Furthermore, macrophage-like cells are observed moving around actively in the Parthenon-like structure and engulfing epithelial cells. The findings in this research are expected to significantly contribute to our understanding of the mechanisms involved in shaping the final structure of the adult tarsus.
摘要：
生物体在形状上表现出显著的多样性。尽管在揭示发育过程中控制细胞命运决定的机制方面已经取得了实质性进展，命运决定的细胞产生生物体最终形状的机制在很大程度上仍然未知。这项研究详细描述了tarsus的最终形状形成的过程，靠近成人腿的远端，在果蝇的蛹期。长达数天的生存成像显示出出乎意料的复杂细胞动力学。上皮细胞暂时形成有趣的结构，我们称之为帕台农神庙状结构。上皮细胞的基底表面和基底膜蛋白的定位最初显示出网状结构，并在帕台农神庙样结构的形成和消失过程中迅速收缩为膜结构。此外，观察到巨噬细胞样细胞在帕台农神庙样结构中活跃地四处移动并吞噬上皮细胞。这项研究的发现预计将大大有助于我们理解形成成年骨最终结构的机制。
