背景/目标:脑脊液(CSF)动力学异常会导致多种疾病,比如脑积水,但是潜在的机制仍然未知。由于缺乏评估系统,尚未建立研究小动物CSF动力学的方法。因此,这项研究的目的是建立时空标记反转脉冲(Time-SLIP)MRI技术,用于评估小鼠的CSF动力学。方法:我们对10只野生型小鼠和20只足尖行走的吉村(TWY)小鼠进行了Time-SLIP技术,后纵韧带骨化(OPLL)的小鼠模型。我们将搅拌距离定义为CSF搅拌的距离并计算平均值±标准偏差。还计算了观察者内部可靠性的组内相关系数。此外,在两次老鼠中,计算搅拌距离与椎管狭窄率(CSR)的相关系数。结果:12周龄和17周龄的TWY小鼠的搅拌距离显着降低(12周龄为1.20±0.16、1.21±0.06和1.21±0.15mm,17周龄为1.32±0.21、1.28±0.23和1.38±0.31mm,B,andC).在TWY小鼠中,三个检查者的内部可靠性优异(>0.90),并且搅拌距离与CSR之间存在强烈的负相关(>-0.80)。结论:在这项研究中,我们在实验小鼠中建立了Time-SLIP技术。该技术可以更好地了解小型实验动物的CSF动力学。
Background/Objectives: Abnormalities in cerebrospinal fluid (CSF) dynamics cause diverse conditions, such as hydrocephalus, but the underlying mechanism is still unknown. Methods to study CSF dynamics in small animals have not been established due to the lack of an evaluation system. Therefore, the purpose of this research study is to establish the time-spatial labeling inversion pulse (Time-SLIP) MRI technique for the evaluation of CSF dynamics in mice. Methods: We performed the Time-SLIP technique on 10 wild-type mice and 20 Tiptoe-walking Yoshimura (TWY) mice, a mouse model of ossification of the posterior longitudinal ligament (OPLL). We defined the stir distance as the distance of CSF stirring and calculated the mean ± standard deviation. The intraclass correlation coefficient of intraobserver reliability was also calculated. Furthermore, in TWY mice, the correlation coefficient between stir distance and canal stenosis ratio (CSR) was calculated. Results: The stir distance was significantly lower in TWY mice at 12 weeks and 17 weeks of age (1.20 ± 0.16, 1.21 ± 0.06, and 1.21 ± 0.15 mm at 12 weeks and 1.32 ± 0.21, 1.28 ± 0.23, and 1.38 ± 0.31 mm at 17 weeks for examiners A, B, and C). The intrarater reliability of the three examiners was excellent (>0.90) and there was a strongly negative correlation between stir distance and CSR in TWY mice (>-0.80). Conclusions: In this study, we established the Time-SLIP technique in experimental mice. This technique allows for a better understanding of CSF dynamics in small laboratory animals.