PDF(Pubmed)
Abstract：
UNASSIGNED: Bee pollen possesses favorable anticancer activities. As a medicinal plant source, Schisandra chinensis bee pollen (SCBP) possesses potential pharmacological properties, such as reducing cisplatin-induced liver injury, but its anti-liver cancer effect is still rarely reported. This paper aims to investigate the effect and mechanism of SCBP extract (SCBPE) on hepatocellular carcinoma HepG2 cells.
UNASSIGNED: The effect of SCBPE on cell proliferation and migration of HepG2 cells was evaluated based on MTT assay, morphology observation, or scratching assay. Furthermore, tandem mass tag-based quantitative proteomics was used to study the effect mechanisms. The mRNA expression levels of identified proteins were verified by RT-qPCR.
UNASSIGNED: Tandem mass tag-based quantitative proteomics showed that 61 differentially expressed proteins were obtained in the SCBPE group compared with the negative-control group: 18 significantly downregulated and 43 significantly upregulated proteins. Bioinformatic analysis showed the significantly enriched KEGG pathways were predominantly ferroptosis-, Wnt-, and hepatocellular carcinoma-signaling ones. Protein-protein interaction network analysis and RT-qPCR validation revealed SCBPE also downregulated the focal adhesion-signaling pathway, which is abrogated by PF-562271, a well-known inhibitor of FAK.
UNASSIGNED: This study confirmed SCBPE suppressed the cell proliferation and migration of hepatocellular carcinoma HepG2 cells, mainly through modulation of ferroptosis-, Wnt-, hepatocellular carcinoma-, and focal adhesion-signaling pathways, providing scientific data supporting adjuvant treatment of hepatocellular carcinoma using SCBP.
UNASSIGNED: The effect of SCBPE on cell proliferation and migration of HepG2 cells was evaluated based on MTT assay, morphology observation, or scratching assay. Furthermore, tandem mass tag-based quantitative proteomics was used to study the effect mechanisms. The mRNA expression levels of identified proteins were verified by RT-qPCR.
UNASSIGNED: Tandem mass tag-based quantitative proteomics showed that 61 differentially expressed proteins were obtained in the SCBPE group compared with the negative-control group: 18 significantly downregulated and 43 significantly upregulated proteins. Bioinformatic analysis showed the significantly enriched KEGG pathways were predominantly ferroptosis-, Wnt-, and hepatocellular carcinoma-signaling ones. Protein-protein interaction network analysis and RT-qPCR validation revealed SCBPE also downregulated the focal adhesion-signaling pathway, which is abrogated by PF-562271, a well-known inhibitor of FAK.
UNASSIGNED: This study confirmed SCBPE suppressed the cell proliferation and migration of hepatocellular carcinoma HepG2 cells, mainly through modulation of ferroptosis-, Wnt-, hepatocellular carcinoma-, and focal adhesion-signaling pathways, providing scientific data supporting adjuvant treatment of hepatocellular carcinoma using SCBP.
摘要：
■蜂花粉具有良好的抗癌活性。作为药用植物来源，五味子蜂花粉（SCBP）具有潜在的药理特性，如减少顺铂诱导的肝损伤，但其抗肝癌作用仍鲜有报道。本文旨在探讨SCBP提取物(SCBPE)对肝癌HepG2细胞的作用及其机制。
■基于MTT法评价SCBPE对HepG2细胞增殖和迁移的影响,形态学观察,或抓挠试验。此外，基于串联质量标签的定量蛋白质组学用于研究效应机制。通过RT-qPCR验证鉴定的蛋白质的mRNA表达水平。
■基于串联质量标签的定量蛋白质组学显示，与阴性对照组相比，SCBPE组获得了61种差异表达的蛋白质：18种显著下调，43种显著上调。生物信息学分析显示显著富集的KEGG途径主要是铁胞嘧啶-，Wnt-,和肝细胞癌信号。蛋白质-蛋白质相互作用网络分析和RT-qPCR验证显示，SCBPE也下调了粘着斑信号通路，由PF-562271废除，这是一种众所周知的FAK抑制剂。
■本研究证实SCBPE抑制肝癌HepG2细胞的增殖和迁移,主要通过铁胞嘧啶的调制，Wnt-,肝细胞癌-，和局灶性粘附信号通路，提供支持使用SCBP辅助治疗肝细胞癌的科学数据。
■基于MTT法评价SCBPE对HepG2细胞增殖和迁移的影响,形态学观察,或抓挠试验。此外，基于串联质量标签的定量蛋白质组学用于研究效应机制。通过RT-qPCR验证鉴定的蛋白质的mRNA表达水平。
■基于串联质量标签的定量蛋白质组学显示，与阴性对照组相比，SCBPE组获得了61种差异表达的蛋白质：18种显著下调，43种显著上调。生物信息学分析显示显著富集的KEGG途径主要是铁胞嘧啶-，Wnt-,和肝细胞癌信号。蛋白质-蛋白质相互作用网络分析和RT-qPCR验证显示，SCBPE也下调了粘着斑信号通路，由PF-562271废除，这是一种众所周知的FAK抑制剂。
■本研究证实SCBPE抑制肝癌HepG2细胞的增殖和迁移,主要通过铁胞嘧啶的调制，Wnt-,肝细胞癌-，和局灶性粘附信号通路，提供支持使用SCBP辅助治疗肝细胞癌的科学数据。
