PDF(Pubmed)
Abstract：
In situ-forming biocompatible hydrogels have great potential in various medical applications. Here, we introduce a pH-responsive, self-healable, and biocompatible hydrogel for cell scaffolds and the development of a tumor spheroid phantom for magnetic resonance imaging. The hydrogel (pMAD) was synthesized via amino-yne click chemistry between poly(2-methacryloyloxyethyl phosphorylcholine-co-2-aminoethylmethacrylamide) and dialkyne polyethylene glycol. Rheology analysis, compressive mechanical testing, and gravimetric analysis were employed to investigate the gelation time, mechanical properties, equilibrium swelling, and degradability of pMAD hydrogels. The reversible enamine and imine bond mechanisms leading to the sol-to-gel transition in acidic conditions (pH ≤ 5) were observed. The pMAD hydrogel demonstrated potential as a cellular scaffold, exhibiting high viability and NIH-3T3 fibroblast cell encapsulation under mild conditions (37 °C, pH 7.4). Additionally, the pMAD hydrogel also demonstrated the capability for in vitro magnetic resonance imaging of glioblastoma tumor spheroids based on the chemical exchange saturation transfer effect. Given its advantages, the pMAD hydrogel emerges as a promising material for diverse biomedical applications, including cell carriers, bioimaging, and therapeutic agent delivery.
摘要：
原位形成的生物相容性水凝胶在各种医学应用中具有巨大的潜力。这里,我们引入了一种pH响应，自我治愈,和用于细胞支架的生物相容性水凝胶以及用于磁共振成像的肿瘤球体模型的开发。水凝胶(pMAD)通过聚(2-甲基丙烯酰氧基乙基磷酰胆碱-co-2-氨基乙基甲基丙烯酰胺)和二炔聚乙二醇之间的氨基-炔点击化学合成。流变学分析,压缩机械测试，采用重量分析法研究凝胶化时间，机械性能，平衡膨胀，和pMAD水凝胶的降解性。观察到在酸性条件（pH≤5）下导致溶胶到凝胶转变的可逆烯胺和亚胺键机理。pMAD水凝胶显示出作为细胞支架的潜力，在温和条件下（37°C，pH7.4)。此外,pMAD水凝胶还证明了基于化学交换饱和转移效应的胶质母细胞瘤肿瘤球体的体外磁共振成像能力。鉴于其优势,pMAD水凝胶成为多种生物医学应用的有前途的材料，包括细胞携带者，生物成像,和治疗剂输送。
