Abstract：
Bombus terrestris are efficient pollinators in forestry and agriculture, with higher cold tolerance than other bees. Yet, their cold tolerance mechanism remains unclear. Aquaporins (AQPs) function as cell membrane proteins facilitating rapid water flow, aiding in osmoregulation. Recent studies highlight the importance of insect AQPs in dehydration and cold stress. Comparative transcriptome analysis of B. terrestris under cold stress revealed up-regulation of four AQPs, indicating their potential role in cold tolerance. Seven AQPs-Eglp1, Eglp2, Eglp3, DRIP, PRIP, Bib, and AQP12L-have been identified in B. terrestris. These are widely expressed in various tissues, particularly in the alimentary canal and Malpighian tubules. Functional analysis of BterAQPs in the Xenopus laevis oocytes expressing system showed distinct water and glycerol selectivity, with BterDrip exhibiting the highest water permeability. Molecular modeling of BterDrip revealed six transmembrane domains, two NPA motifs, and an ar/R constriction region (Phe131, His256, Ser265, and Arg271), likely contributing to its water selectivity. Silencing BterDRIP accelerated mortality in B. terrestris under cold stress, highlighting the crucial role of BterDRIP in their cold tolerance and providing a molecular mechanism for their cold adaptation.
摘要：
松柏是林业和农业中有效的授粉者，比其他蜜蜂具有更高的耐寒性。然而，其耐寒机制尚不清楚。水通道蛋白（AQP）作为细胞膜蛋白促进快速水流，帮助渗透调节。最近的研究强调了昆虫AQP在脱水和寒冷胁迫中的重要性。冷应激条件下的白棘芽孢杆菌的比较转录组分析揭示了四个AQP的上调，表明它们在耐寒性中的潜在作用。七个AQP-Eglp1，Eglp2，Eglp3，DRIP，PRIP,围兜,和AQP12L-已被鉴定为沙棘。这些在各种组织中广泛表达，特别是在消化道和马尔皮根小管中。对非洲爪狼卵母细胞表达系统中BterAQPs的功能分析显示出不同的水和甘油选择性，BterDrip具有最高的透水性。BterDrip的分子建模揭示了六个跨膜结构域，两个NPA图案，和AR/R收缩区域（Phe131，His256，Ser265和Arg271），可能有助于其水的选择性。沉默BterDRIP可加速冷应激条件下的白棘芽孢杆菌死亡率，强调BterDRIP在其耐寒性中的关键作用，并为其冷适应提供分子机制。
