PDF(Pubmed)
Abstract：
Bioorthogonal click chemistry has played a transformative role in many research fields, including chemistry, biology, and medicine. Click reactions are crucial to produce increasingly complex bioconjugates, to visualize and manipulate biomolecules in living systems and for various applications in bioengineering and drug delivery. As biological (model) systems grow more complex, researchers have an increasing need for using multiple orthogonal click reactions simultaneously. In this review, we will introduce the most common bioorthogonal reactions and discuss their orthogonal use on the basis of their mechanism and electronic or steric tuning. We provide an overview of strategies to create reaction orthogonality and show recent examples of mutual orthogonal chemistry used for simultaneous biomolecule labeling. We end by discussing some considerations for the type of chemistry needed for labeling biomolecules in a system of choice.
摘要：
生物正交点击化学在许多研究领域发挥了变革性的作用，包括化学,生物学和医学。点击反应对于产生越来越复杂的生物缀合物至关重要，可视化和操纵生物分子在生命系统和生物工程和药物输送的各种应用。随着生物（模型）系统变得越来越复杂，研究人员越来越需要同时使用多个正交点击反应。在这次审查中,我们将介绍最常见的生物正交反应，并根据其机理和电子或空间调谐讨论其正交用途。我们提供了创建反应正交性的策略的概述，并展示了用于同时生物分子标记的相互正交化学的最新实例。最后，我们讨论了在选择的系统中标记生物分子所需的化学类型的一些考虑因素。
