Abstract：
Spent hemp biomass (SHB) contains trace amounts of cannabinoids, including Δ9-tetrahydrocannabinol (Δ9-THC), that may accumulate in the tissues of animals consuming SHB. We measured cannabinoid residues in the liver, adipose tissue, and muscle of finishing lambs fed either 10% or 20% SHB for 8 weeks, or 4 weeks followed by 4 weeks SHB withdrawal. We detected multiple cannabinoids in the liver at a similar proportion to the SHB. However, CBD and Δ9-THC were enriched >20-fold in the adipose and muscle, compared to their proportion in SHB. The highest concentration of Δ9-THC was detected in adipose tissue and was 7.4-times higher than in muscle. Most cannabinoids were undetectable in tissues after 4 weeks of clearance. The consumers\' exposure assessment on Δ9-THC revealed tissue levels of total THC (THCA+Δ9-THC) that exceed the acute reference dose of 1 μg/kg BW across population groups. When consuming meat from the lambs fed 10% and 20% SHB, the maximum total THC exposure was 2.03 and 7.32 μg/kg BW, respectively, equal to or below the Lowest Observed Adverse Effect Level of 36 μg/kg BW, the No Observed Adverse Effect Level of 12 μg/kg BW or a tolerable dose intake of 7 μg/kg BW.
摘要：
用过的大麻生物质（SHB）含有痕量的大麻素，包括Δ9-四氢大麻酚（Δ9-THC），可能在食用SHB的动物组织中积累。我们测量了肝脏中的大麻残留，脂肪组织,和饲喂10％或20％SHB的肥羊肌肉8周，或4周，随后4周SHB停药。我们在肝脏中检测到与SHB相似比例的多种大麻素。然而,CBD和Δ9-THC在脂肪和肌肉中富集>20倍，与它们在SHB中的比例相比。在脂肪组织中检测到Δ9-THC的最高浓度，是肌肉中的7.4倍。清除4周后，大多数大麻素在组织中无法检测到。消费者对Δ9-THC的暴露评估显示，总THC（THCAΔ9-THC）的组织水平超过了人群中1μg/kgBW的急性参考剂量。当食用饲喂10%和20%SHB的羔羊肉时，最大总THC暴露量为2.03和7.32μg/kgBW，分别,等于或低于最低观察到的36μg/kgBW的不良反应水平，未观察到12μg/kg体重的不良反应水平或7μg/kg体重的耐受剂量摄入量。
