Abstract：
BACKGROUND: Dermatophagoides pteronyssinus and Dermatophagoides farinae belong to the family Pyroglyphidae (subfamily: \"Dermatophagoidinae\") and have the respective allergenic proteins of Der p1, Der p2, and Der p23 and Der f1 and Der f2. Euroglyphus maynei, belongs to the family Pyroglyphidae (subfamily: \"Pyroglyphinae\") and its main allergenic protein is Eur m1, a source of sensitization. Sensitization to D. pteronyssinus and D. farinae is assessed through skin tests, while sensitization to E. maynei is assessed less frequently.
OBJECTIVE: This experimental work aims to analyze the prevalence of sensitization to E. maynei in patients with respiratory allergies treated at M. Albanesi Allergy and Immunology Unit in Bari, Italy, and the sequence homology of major allergenic proteins of E. maynei with D. farinae and D. pteronyssinus was analyzed.
METHODS: In this real-life study, 65 patients were enrolled. In particular, patients with respiratory allergy were subjected to skin prick tests for common respiratory allergens, including Euroglyphus maynei. The sequence homology analysis was performed between the major allergenic proteins of E. maynei and those of D. pteronyssinus and D. farinae.
RESULTS: Sensitization to E. maynei accounts for 41.5% of patients. All patients with E. maynei sensitization had concomitant sensitization to D. farinae and D. pteronyssinus. The analysis of sequence homology of Der p1 and Der f1 proteins with the sequence of Eur m1 protein demonstrated an identity of 84.4% and 86%, respectively.
CONCLUSIONS: Nearly 50% of house dust mites-sensitized patients have a concomitant sensitization to E. maynei. The cross-sensitization could be due to Der f1, Der p1, and Eur m1 similarity.
OBJECTIVE: This experimental work aims to analyze the prevalence of sensitization to E. maynei in patients with respiratory allergies treated at M. Albanesi Allergy and Immunology Unit in Bari, Italy, and the sequence homology of major allergenic proteins of E. maynei with D. farinae and D. pteronyssinus was analyzed.
METHODS: In this real-life study, 65 patients were enrolled. In particular, patients with respiratory allergy were subjected to skin prick tests for common respiratory allergens, including Euroglyphus maynei. The sequence homology analysis was performed between the major allergenic proteins of E. maynei and those of D. pteronyssinus and D. farinae.
RESULTS: Sensitization to E. maynei accounts for 41.5% of patients. All patients with E. maynei sensitization had concomitant sensitization to D. farinae and D. pteronyssinus. The analysis of sequence homology of Der p1 and Der f1 proteins with the sequence of Eur m1 protein demonstrated an identity of 84.4% and 86%, respectively.
CONCLUSIONS: Nearly 50% of house dust mites-sensitized patients have a concomitant sensitization to E. maynei. The cross-sensitization could be due to Der f1, Der p1, and Eur m1 similarity.
摘要：
背景：翼状螨和粉尘螨属于Pyroglyphidae家族（“Dermaticoidinae”亚家族），分别具有Derp1，Derp2和Derp23以及Derf1和Derf2的变应原蛋白。欧照梅尼，属于Pyroglyphidae家族（亚家族：“Pyroglyphinae”），其主要致敏蛋白是Eurm1，一种致敏来源。通过皮肤试验评估对D.pteronysinus和D.farinae的敏感性，而对E.maynei的敏感性评估较少。
目的：这项实验工作旨在分析在巴里的M.Albanesi过敏和免疫学部门治疗的呼吸道过敏患者对E.maynei的致敏率，意大利,并分析了E.maynei与D.farinae和D.pteronysinus的主要致敏蛋白的序列同源性。
方法：在这项现实生活中的研究中，65例患者入组。特别是,呼吸道过敏患者接受了常见呼吸道过敏原的皮肤点刺试验，包括欧彩玛尼.在E.maynei的主要过敏原蛋白与D.pteronysinus和D.farinae的主要过敏原蛋白之间进行序列同源性分析。
结果：对E.maynei敏感的患者占41.5%。所有患有E.maynei致敏的患者均对D.farinae和D.pteronysinus致敏。Derp1和Derf1蛋白与Eurm1蛋白序列的序列同源性分析显示出84.4%和86%的同一性,分别。
结论：近50%的屋尘螨致敏患者对E.maynei有伴随致敏作用。交叉敏化可能是由于Derf1、Derp1和Eurm1相似性。
目的：这项实验工作旨在分析在巴里的M.Albanesi过敏和免疫学部门治疗的呼吸道过敏患者对E.maynei的致敏率，意大利,并分析了E.maynei与D.farinae和D.pteronysinus的主要致敏蛋白的序列同源性。
方法：在这项现实生活中的研究中，65例患者入组。特别是,呼吸道过敏患者接受了常见呼吸道过敏原的皮肤点刺试验，包括欧彩玛尼.在E.maynei的主要过敏原蛋白与D.pteronysinus和D.farinae的主要过敏原蛋白之间进行序列同源性分析。
结果：对E.maynei敏感的患者占41.5%。所有患有E.maynei致敏的患者均对D.farinae和D.pteronysinus致敏。Derp1和Derf1蛋白与Eurm1蛋白序列的序列同源性分析显示出84.4%和86%的同一性,分别。
结论：近50%的屋尘螨致敏患者对E.maynei有伴随致敏作用。交叉敏化可能是由于Derf1、Derp1和Eurm1相似性。
