Abstract：
This study explored the effects of 1, 2-bis (2,4, 6-tribromophenoxy) ethane (BTBPE) and bis (2-ethylhexyl) tetrabromophthalate (TBPH) on serum metabolites and lipids in male Sprague-Dawley (SD) rats. Rats were orally gavaged 250 mg/kg bw of BTBPE and 500 mg/kg bw of TBPH for 28 consecutive days. Serum samples were collected for metabolomics and lipidomics analysis. Orthogonal partial least squares discriminant analysis (OPLS-DA) was used to explore changes in rat metabolic patterns. Least absolute shrinkage and selection operator (LASSO) regression models were established using serum levels of total thyroxine (TT4), free thyroxine (FT4), and rats\' grouping information as variables to screen for robust differential substances. SuperPred was the database to obtain potential targets. The metabolomics and lipidomics results showed that BTBPE and TBPH had an impact on rat metabolic patterns, affecting pathways such as vitamin B6 synthesis. For BTBPE treatment, pyridoxal and ceramide (Cer) 24:0;4O were selected as differential substances related to thyroid hormones. For TBPH treatment, dehydroascorbic acid, acylcarnitine (CAR) 19:0, and diglyceride (DG) 38:4 were selected as differential substances related to thyroid hormones. Serotonin 2c receptor and cyclooxygenase-2 were chosen as potential targets of BTBPE and TBPH, respectively. In conclusion, this study found that BTBPE and TBPH impacted the metabolism of rats, and this effect may be related to changes in thyroid function.
摘要：
本研究探讨了1，2-双（2，4，6-三溴苯氧基）乙烷（BTBPE）和双（2-乙基己基）四溴苯酸盐（TBPH）对雄性SD大鼠血清代谢产物和脂质的影响。大鼠连续28天口服250mg/kgbw的BTBPE和500mg/kgbw的TBPH。收集血清样品用于代谢组学和脂质组学分析。正交偏最小二乘判别分析（OPLS-DA）用于探索大鼠代谢模式的变化。使用血清总甲状腺素（TT4）水平建立最小绝对收缩和选择算子（LASSO）回归模型，游离甲状腺素（FT4），和大鼠将信息分组为变量，以筛选稳健的差异物质。SuperPred是获得潜在目标的数据库。代谢组学和脂质组学结果表明BTBPE和TBPH对大鼠代谢模式有影响,影响维生素B6合成等途径。对于BTBPE治疗，吡哆醛和神经酰胺（Cer）24：0；选择4O作为与甲状腺激素有关的差异物质。对于TBPH处理，脱氢抗坏血酸，选择酰基肉碱（CAR）19：0和甘油二酯（DG）38：4作为与甲状腺激素有关的差异物质。选择5-羟色胺2c受体和环氧合酶-2作为BTBPE和TBPH的潜在靶标，分别。总之,本研究发现BTBPE和TBPH影响大鼠的代谢,这种效应可能与甲状腺功能的变化有关。
