PDF(Pubmed)
Abstract：
DNA origami nanostructures (DOs) are promising tools for applications including drug delivery, biosensing, detecting biomolecules, and probing chromatin substructures. Targeting these nanodevices to mammalian cell nuclei could provide impactful approaches for probing, visualizing, and controlling biomolecular processes within live cells. We present an approach to deliver DOs into live-cell nuclei. We show that these DOs do not undergo detectable structural degradation in cell culture media or cell extracts for 24 hours. To deliver DOs into the nuclei of human U2OS cells, we conjugated 30-nanometer DO nanorods with an antibody raised against a nuclear factor, specifically the largest subunit of RNA polymerase II (Pol II). We find that DOs remain structurally intact in cells for 24 hours, including inside the nucleus. We demonstrate that electroporated anti-Pol II antibody-conjugated DOs are piggybacked into nuclei and exhibit subdiffusive motion inside the nucleus. Our results establish interfacing DOs with a nuclear factor as an effective method to deliver nanodevices into live-cell nuclei.
摘要：
DNA折纸纳米结构（DOs）是包括药物递送在内的应用的有前途的工具，生物传感，检测生物分子，探测染色质亚结构。将这些纳米设备靶向哺乳动物细胞核可以为探测提供有效的方法，可视化，并控制活细胞内的生物分子过程。我们提出了一种将DO递送到活细胞核中的方法。我们表明，这些DO在细胞培养基或细胞提取物中24小时内不会发生可检测的结构降解。为了将DOs传递到人类U2OS细胞的细胞核中，我们将30纳米DO纳米棒与针对核因子的抗体结合起来，特别是RNA聚合酶II（PolII）的最大亚基。我们发现DO在细胞中保持结构完整24小时，包括在细胞核内部。我们证明了电穿孔的抗PolII抗体缀合的DOs被搭载到细胞核中，并在细胞核内表现出亚扩散运动。我们的结果建立了具有核因子的接口DO作为将纳米设备递送到活细胞核的有效方法。
