PDF(Pubmed)
Abstract：
BACKGROUND: This study investigated changes in plasma microbial-derived extracellular vesicles (EVs) in patients with polycystic ovary syndrome and insulin resistance (PCOS-IR) before and after metformin treatment, and aimed to identify bacterial taxa within EVs that were biologically and statistically significant for diagnosis and treatment.
METHODS: The case-control study was conducted at Xiamen Chang Gung Hospital, Hua Qiao University. Plasma samples were collected from five PCOS-IR patients of childbearing age before and after 3 months of metformin treatment, and the samples were sequenced. The diversity and taxonomic composition of different microbial communities were analyzed through full-length 16 S glycosomal RNA gene sequencing.
RESULTS: After metformin treatment, fasting plasma glucose levels and IR degree of PCOS-IR patients were significantly improved. The 16 S analysis of plasma EVs from metformin-treated patients showed higher microbial diversity. There were significant differences in EVs derived from some environmental bacteria before and after metformin treatment. Notably, Streptococcus salivarius was more abundant in the metformin-treated group, suggesting it may be a potential probiotic.
CONCLUSIONS: The study demonstrated changes in the microbial composition of plasma EVs before and after metformin treatment. The findings may offer new insights into the pathogenesis of PCOS-IR and provide new avenues for research.
METHODS: The case-control study was conducted at Xiamen Chang Gung Hospital, Hua Qiao University. Plasma samples were collected from five PCOS-IR patients of childbearing age before and after 3 months of metformin treatment, and the samples were sequenced. The diversity and taxonomic composition of different microbial communities were analyzed through full-length 16 S glycosomal RNA gene sequencing.
RESULTS: After metformin treatment, fasting plasma glucose levels and IR degree of PCOS-IR patients were significantly improved. The 16 S analysis of plasma EVs from metformin-treated patients showed higher microbial diversity. There were significant differences in EVs derived from some environmental bacteria before and after metformin treatment. Notably, Streptococcus salivarius was more abundant in the metformin-treated group, suggesting it may be a potential probiotic.
CONCLUSIONS: The study demonstrated changes in the microbial composition of plasma EVs before and after metformin treatment. The findings may offer new insights into the pathogenesis of PCOS-IR and provide new avenues for research.
摘要：
背景：这项研究调查了多囊卵巢综合征和胰岛素抵抗（PCOS-IR）患者在二甲双胍治疗前后血浆微生物来源的细胞外囊泡（EVs）的变化，目的是确定电动汽车中对诊断和治疗具有生物学和统计学意义的细菌分类群。
方法：病例对照研究在厦门长庚医院进行，华桥大学。收集5例育龄期PCOS-IR患者二甲双胍治疗前和治疗3个月后的血浆样本,并对样品进行测序。通过全长16S糖体RNA基因测序分析了不同微生物群落的多样性和分类组成。
结果：二甲双胍治疗后，PCOS-IR患者空腹血糖水平和IR程度均有明显改善。来自二甲双胍治疗患者的血浆EV的16S分析显示出更高的微生物多样性。在二甲双胍治疗前后，来自某些环境细菌的EV存在显着差异。值得注意的是,唾液链球菌在二甲双胍治疗组中更丰富，这表明它可能是一种潜在的益生菌。
结论：该研究证实了二甲双胍治疗前后血浆EV微生物组成的变化。这些发现可能为PCOS-IR的发病机制提供新的见解，并为研究提供新的途径。
方法：病例对照研究在厦门长庚医院进行，华桥大学。收集5例育龄期PCOS-IR患者二甲双胍治疗前和治疗3个月后的血浆样本,并对样品进行测序。通过全长16S糖体RNA基因测序分析了不同微生物群落的多样性和分类组成。
结果：二甲双胍治疗后，PCOS-IR患者空腹血糖水平和IR程度均有明显改善。来自二甲双胍治疗患者的血浆EV的16S分析显示出更高的微生物多样性。在二甲双胍治疗前后，来自某些环境细菌的EV存在显着差异。值得注意的是,唾液链球菌在二甲双胍治疗组中更丰富，这表明它可能是一种潜在的益生菌。
结论：该研究证实了二甲双胍治疗前后血浆EV微生物组成的变化。这些发现可能为PCOS-IR的发病机制提供新的见解，并为研究提供新的途径。
