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Abstract：
The use of novel active ingredients for the functional modification of chitosan nanoformulations has attracted global attention. In this study, chitosan has been functionalized via histidine to craft novel chitosan-histidine nanoformulation (C-H NF) using ionic gelation method. C-H NF exhibited elite physico-biochemical properties, influencing physiological and biochemical dynamics in Tomato. These elite properties include homogenous-sized nanoparticles (314.4 nm), lower PDI (0.218), viscosity (1.43 Cps), higher zeta potential (11.2 mV), nanoparticle concentration/ml (3.53 × 108), conductivity (0.046 mS/cm), encapsulation efficiency (53%), loading capacity (24%) and yield (32.17%). FTIR spectroscopy revealed histidine interaction with C-H NF, while SEM and TEM exposed its porous structure. Application of C-H NF to Tomato seedling and potted plants through seed treatment and foliar spray positively impacts growth parameters, antioxidant-defense enzyme activities, reactive oxygen species (ROS) content, and chlorophyll and nitrogen content. We claim that the histidine-functionalized chitosan nanoformulation enhances physico-biochemical properties, highlighting its potential to elevate biochemical and physiological processes of Tomato plant.
摘要：
利用新型活性成分对壳聚糖纳米制剂进行功能改性已引起全球关注。在这项研究中,壳聚糖已通过组氨酸功能化，以使用离子凝胶法制备新型壳聚糖-组氨酸纳米制剂（C-HNF）。C-HNF表现出优良的物理生化特性，影响番茄生理生化动态。这些优良的特性包括均匀尺寸的纳米颗粒（314.4nm），较低的PDI（0.218），粘度（1.43Cps），较高的zeta电位（11.2mV），纳米粒子浓度/ml（3.53×108），电导率（0.046mS/cm），封装效率(53%)，装载能力(24%)和产量(32.17%)。FTIR光谱显示组氨酸与C-HNF，而SEM和TEM暴露了其多孔结构。通过种子处理和叶面喷雾将C-HNF应用于番茄幼苗和盆栽植物，对生长参数产生积极影响。抗氧化防御酶活性，活性氧（ROS）含量，叶绿素和氮含量。我们声称组氨酸官能化的壳聚糖纳米制剂增强了物理生化特性，强调其提高番茄植物生化和生理过程的潜力。
