Abstract：
H2S plays a crucial role in numerous physiological and pathological processes. In this project, a new fluorescent probe, SG-H2S, for the detection of H2S, was developed by introducing the recognition group 2,4-dinitrophenyl ether. The combination of rhodamine derivatives can produce both colorimetric reactions and fluorescence reactions. Compared with the current H2S probes, the main advantages of SG-H2S are its wide pH range (5-9), fast response (30 min), and high selectivity in competitive species (including biological mercaptan). The probe SG-H2S has low cytotoxicity and has been successfully applied to imaging in MCF-7 cells, HeLa cells, and BALB/c nude mice. We hope that SG-H2S will provide a vital method for the field of biology.
摘要：
H2S在许多生理和病理过程中起着至关重要的作用。在这个项目中,一种新的荧光探针,SG-H2S,用于检测H2S，是通过引入识别基团2,4-二硝基苯醚开发的。罗丹明衍生物的组合可以产生比色反应和荧光反应。与目前的H2S探头相比，SG-H2S的主要优点是其宽pH范围(5-9)，快速响应（30分钟），和高选择性的竞争性物种（包括生物硫醇）。SG-H2S探针具有较低的细胞毒性,已成功应用于MCF-7细胞的成像,HeLa细胞,和BALB/c裸鼠。我们希望SG-H2S将为生物学领域提供重要的方法。
