Abstract：
Marine ecosystems are under escalating threats from myriad environmental stressors, necessitating a deeper understanding of their impact on biodiversity and the health of sentinel organisms. In this study, we carried out a spatiotemporal multi-omic analysis of liquid biopsies collected from mussels (Mytilus spp.) in marine ecosystems of a national park. We delved into the epigenomic, transcriptomic, glycomic, proteomic, and microbiomic profiles to unravel the intricate interplay between ecosystem biodiversity and mussels\' biological response to their environments. Our analysis revealed temporal fluctuations in the alpha diversity of the circulating microbiome associated with human activities. Analysis of the hemolymphatic circulating cell-free DNA (ccfDNA) provided information on the biodiversity and the presence of potential pathogens. Epigenomic analysis revealed widespread hypomethylation sites within the mitochondrial (mtDNA). Comparative transcriptomic and glycomic analyses highlighted differences in metabolic pathways and genes associated with immune and wound healing functions. This study demonstrates the potential of multi-omic analysis of liquid biopsy in sentinel to provide a holistic view of human activities\' environmental impacts on marine coastal ecosystems. Overall, this approach has the potential to enhance the effectiveness and efficiency of various conservation efforts, leading to more informed decision-making and better outcomes for biodiversity and ecosystem conservation.
摘要：
海洋生态系统正受到无数环境压力因素的不断升级的威胁，需要更深入地了解它们对生物多样性和前哨生物健康的影响。在这项研究中,我们对从贻贝（Mytilusspp。)在国家公园的海洋生态系统中。我们深入研究了表观基因组，转录组，glycomic,蛋白质组学，和微生物组学概况，以解开生态系统生物多样性与贻贝对其环境的生物反应之间的复杂相互作用。我们的分析揭示了与人类活动相关的循环微生物组的α多样性的时间波动。血淋巴循环无细胞DNA（ccfDNA）的分析提供了有关生物多样性和潜在病原体存在的信息。表观基因组分析揭示了线粒体（mtDNA）内广泛的低甲基化位点。比较转录组和糖组学分析强调了与免疫和伤口愈合功能相关的代谢途径和基因的差异。这项研究证明了前哨液体活检的多组学分析的潜力，可以提供人类活动对海洋沿海生态系统的环境影响的整体观点。总的来说,这种方法有可能提高各种保护工作的有效性和效率，为生物多样性和生态系统保护提供更明智的决策和更好的结果。
