Abstract：
The biofilm-mediated implant infections pose a huge threat to human health. It is urgent to explore strategies to reverse this situation. Herein, we design 3-amino-1,2,4-triazole-5-thiol (ATT)-modified gold nanoclusters (AGNCs) to realize biofilm-targeting and near-infrared (NIR)-II light-responsive antibiofilm therapy. The AGNCs can interact with the bacterial extracellular DNA through the formation of hydrogen bonds between the amine groups on the ATT and the hydroxyl groups on the DNA. The AGNCs show photothermal properties even at a low power density (0.5 W/cm2) for a short-time (5 min) irradiation, making them highly effective in eradicating the biofilm with a dispersion rate up to 90 %. In vivo infected catheter implantation model demonstrates an exceptional high ability of the AGNCs to eradicate approximately 90 % of the bacteria encased within the biofilms. Moreover, the AGNCs show no detectable toxicity or systemic effects in mice. Our study suggests the great potential of the AGNCs for long-term prevention and elimination of the biofilm-mediated infections.
摘要：
生物膜介导的植入物感染对人类健康构成巨大威胁。迫切需要探索扭转这种局面的战略。在这里，我们设计了3-氨基-1,2,4-三唑-5-硫醇（ATT）修饰的金纳米簇（AGNs），以实现生物膜靶向和近红外（NIR）-II光响应性抗生物膜治疗。AGNC可以通过在ATT上的胺基和DNA上的羟基之间形成氢键与细菌胞外DNA相互作用。即使在短时间（5分钟）照射的低功率密度（0.5W/cm2）下，AGNCs也显示出光热特性，使它们在消除生物膜方面非常有效，分散率高达90％。体内感染的导管植入模型证明了AGNC根除生物膜内包裹的大约90%的细菌的异常高的能力。此外，AGNC在小鼠中没有可检测到的毒性或全身性作用。我们的研究表明，AGNCs具有长期预防和消除生物膜介导的感染的巨大潜力。
