关键词: Diterpenes Eleutherobin Octocoral terpenes Saccharomyces expression Soft corals Terpene cyclase

Mesh : Anthozoa / enzymology metabolism genetics Terpenes / metabolism chemistry Animals Saccharomyces cerevisiae / genetics metabolism Escherichia coli / genetics metabolism Alkyl and Aryl Transferases / metabolism genetics

来  源:   DOI:10.1016/bs.mie.2024.03.023

Abstract:
Coral terpenes are important molecules with numerous applications. Here, we describe a robust and simple method to produce coral terpene scaffolds at scale. As an example of the approach, here we discover, express, and characterize further klysimplexin R synthases, expanding the known enzymology of soft coral terpene cyclases. We hope that the underlying method described will enable widespread basic research into the functions of coral terpenes and their biosynthetic genes, as well as the commercial development of biomedically and technologically important molecules.
摘要:
珊瑚萜烯是具有许多应用的重要分子。这里,我们描述了一种强大而简单的方法来大规模生产珊瑚萜烯支架。作为该方法的一个例子,在这里我们发现,快递,并进一步表征klysimplexinR合成酶,扩展已知的软珊瑚萜烯环化酶的酶学。我们希望所描述的基本方法将能够对珊瑚萜烯及其生物合成基因的功能进行广泛的基础研究,以及生物医学和技术重要分子的商业开发。
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