Abstract：
BACKGROUND: The roles of IL-10, IL-11, COX-2, BCL6, ZEB1, and ZEB2 genes in the potential correlation between polycystic ovary syndrome (PCOS), inflammation, and cancer remain controversial.
OBJECTIVE: This study aimed to compare serum levels of IL-10 and IL-11 and gene expression of IL-10, IL-11, COX-2, BCL6, ZEB1, and ZEB2 in PBMCs of women with PCOS and healthy controls.
METHODS: A case-control study included 40 women with PCOS as the case group and 40 healthy women as controls. Group matching for age and BMI was performed. Serum levels of IL-10 and IL-11 were assessed using ELISA, while gene expression was measured using real-time PCR. Parameters were compared between groups, and correlations among gene expression and serum levels were explored.
RESULTS: In comparison to healthy women, women with PCOS exhibited a significant decrease in the expression of COX-2 and IL-10 genes (p<0.001), alongside a significant increase in ZEB2 gene expression (p<0.001). There were no significant differences observed in the expression of IL-11, BCL6, and ZEB1 genes. Furthermore, the serum level of IL-10 was significantly lower in women with PCOS compared to the control group (p<0.001), while no significant difference was found in IL-11 levels. Additionally, no significant correlations were identified between gene expression and serum levels.
CONCLUSIONS: In women with PCOS, reduced IL-10 gene expression may indicate inflammation and serve as a diagnostic biomarker. However, conflicting findings on COX-2 expression complicate understanding. Elevated ZEB2 expression in PCOS women may lead to infertility, epithelial-mesenchymal transition, and aggressive phenotypes.
OBJECTIVE: This study aimed to compare serum levels of IL-10 and IL-11 and gene expression of IL-10, IL-11, COX-2, BCL6, ZEB1, and ZEB2 in PBMCs of women with PCOS and healthy controls.
METHODS: A case-control study included 40 women with PCOS as the case group and 40 healthy women as controls. Group matching for age and BMI was performed. Serum levels of IL-10 and IL-11 were assessed using ELISA, while gene expression was measured using real-time PCR. Parameters were compared between groups, and correlations among gene expression and serum levels were explored.
RESULTS: In comparison to healthy women, women with PCOS exhibited a significant decrease in the expression of COX-2 and IL-10 genes (p<0.001), alongside a significant increase in ZEB2 gene expression (p<0.001). There were no significant differences observed in the expression of IL-11, BCL6, and ZEB1 genes. Furthermore, the serum level of IL-10 was significantly lower in women with PCOS compared to the control group (p<0.001), while no significant difference was found in IL-11 levels. Additionally, no significant correlations were identified between gene expression and serum levels.
CONCLUSIONS: In women with PCOS, reduced IL-10 gene expression may indicate inflammation and serve as a diagnostic biomarker. However, conflicting findings on COX-2 expression complicate understanding. Elevated ZEB2 expression in PCOS women may lead to infertility, epithelial-mesenchymal transition, and aggressive phenotypes.
摘要：
背景：IL-10，IL-11，COX-2，BCL6，ZEB1和ZEB2基因在多囊卵巢综合征（PCOS）之间的潜在相关性中的作用，炎症,癌症仍然存在争议。
目的：本研究旨在比较PCOS女性和健康对照组PBMC中IL-10和IL-11的血清水平以及IL-10，IL-11，COX-2，BCL6，ZEB1和ZEB2的基因表达。
方法：病例对照研究包括40例PCOS妇女作为病例组，40例健康妇女作为对照。进行年龄和BMI的分组匹配。使用ELISA评估血清IL-10和IL-11的水平，同时使用实时PCR测量基因表达。组间比较参数，并探讨了基因表达与血清水平之间的相关性。
结果：与健康女性相比，患有PCOS的女性表现出COX-2和IL-10基因表达的显着下降（p<0.001），ZEB2基因表达显著增加（p<0.001）。在IL-11、BCL6和ZEB1基因的表达中没有观察到显著差异。此外，与对照组相比，PCOS女性的血清IL-10水平显着降低（p<0.001），而IL-11水平无显著差异。此外,在基因表达和血清水平之间没有发现显著的相关性.
结论：在患有PCOS的女性中，IL-10基因表达降低可能提示炎症,并可作为诊断生物标志物.然而,关于COX-2表达的矛盾发现使理解复杂化。在PCOS女性中ZEB2表达升高可能导致不孕,上皮-间质转化，和侵略性表型。
目的：本研究旨在比较PCOS女性和健康对照组PBMC中IL-10和IL-11的血清水平以及IL-10，IL-11，COX-2，BCL6，ZEB1和ZEB2的基因表达。
方法：病例对照研究包括40例PCOS妇女作为病例组，40例健康妇女作为对照。进行年龄和BMI的分组匹配。使用ELISA评估血清IL-10和IL-11的水平，同时使用实时PCR测量基因表达。组间比较参数，并探讨了基因表达与血清水平之间的相关性。
结果：与健康女性相比，患有PCOS的女性表现出COX-2和IL-10基因表达的显着下降（p<0.001），ZEB2基因表达显著增加（p<0.001）。在IL-11、BCL6和ZEB1基因的表达中没有观察到显著差异。此外，与对照组相比，PCOS女性的血清IL-10水平显着降低（p<0.001），而IL-11水平无显著差异。此外,在基因表达和血清水平之间没有发现显著的相关性.
结论：在患有PCOS的女性中，IL-10基因表达降低可能提示炎症,并可作为诊断生物标志物.然而,关于COX-2表达的矛盾发现使理解复杂化。在PCOS女性中ZEB2表达升高可能导致不孕,上皮-间质转化，和侵略性表型。
