Abstract：
Root metaproteome analysis can reveal the functions that govern plant-microbe and microbe-microbe interactions under specific environmental conditions. Efficient protein extraction method from microbes associated with plant roots is crucial for the comprehensive analysis of the metaproteome. In this chapter, a straightforward protein extraction method for roots of Arabidopsis inoculated with a microbial community that uses only milligrams of tissue is outlined. In addition, the plant inoculation using a synthetic community (SynCom) and the methods for a nanoflow liquid chromatography coupled to a high-resolution/high-accuracy mass spectrometer (LC-MS/MS) are described.
摘要：
根元蛋白质组分析可以揭示在特定环境条件下控制植物-微生物和微生物-微生物相互作用的功能。从与植物根相关的微生物中高效提取蛋白质的方法对于综合分析蛋白质组至关重要。在这一章中,概述了一种简单的蛋白质提取方法，该方法用于接种了仅使用毫克组织的微生物群落的拟南芥根。此外,描述了使用合成群落（SynCom）的植物接种以及与高分辨率/高精度质谱仪（LC-MS/MS）耦合的纳流液相色谱的方法。
