%0 Journal Article
%T A Complete Metaproteomic Workflow for Arabidopsis Roots Inoculated by Synthetic Bacteria.
%A Salvato F
%A Kleiner M
%J Methods Mol Biol
%V 2820
%N 0
%D 2024
%M 38941015
暂无%R 10.1007/978-1-0716-3910-8_7
%X Root metaproteome analysis can reveal the functions that govern plant-microbe and microbe-microbe interactions under specific environmental conditions. Efficient protein extraction method from microbes associated with plant roots is crucial for the comprehensive analysis of the metaproteome. In this chapter, a straightforward protein extraction method for roots of Arabidopsis inoculated with a microbial community that uses only milligrams of tissue is outlined. In addition, the plant inoculation using a synthetic community (SynCom) and the methods for a nanoflow liquid chromatography coupled to a high-resolution/high-accuracy mass spectrometer (LC-MS/MS) are described.