Abstract：
Due to technological advances in mass spectrometry, significant progress has been achieved recently in plant hormone research. Nowadays, plant hormonomics is well established as a fully integrated scientific field focused on the analysis of phytohormones, mainly on their isolation, identification and spatiotemporal quantification in plants. This review represents a comprehensive meta-study of the advances in the phytohormone analysis by mass spectrometry over the past decade. To address current trends and future perspectives, Web of Science data were systematically collected and key features such as mass spectrometry-based analyses were evaluated using multivariate data analysis methods. Our findings showed that plant hormonomics is currently divided into targeted and untargeted approaches. Both aim to miniaturize the sample, allowing high-resolution quantification to be covered in plant organs as well as subcellular compartments. Therefore, we can study plant hormone biosynthesis, metabolism and signalling at a spatio-temporal resolution. Moreover, this trend has recently been accelerated by technological advances such as fluorescence-activated cell sorting or mass spectrometry imaging.
摘要：
由于质谱技术的进步,植物激素研究最近取得了重大进展。如今,植物激素组学是一个完全整合的科学领域，专注于植物激素的分析，主要是因为他们与世隔绝，植物的鉴定和时空定量。这篇综述代表了过去十年中通过质谱进行植物激素分析的进展的全面荟萃研究。为了应对当前趋势和未来前景，系统收集了WebofScience数据，并使用多变量数据分析方法评估了关键特征，例如基于质谱的分析。我们的发现表明，植物激素组学目前分为靶向和非靶向方法。两者都旨在使样本小型化，允许高分辨率定量被覆盖在植物器官以及亚细胞区室中。因此,我们可以研究植物激素的生物合成，时空分辨率下的代谢和信号传导。此外，这种趋势最近已经加速了技术进步，如荧光激活细胞分选或质谱成像。
