背景:异戊烯基转移酶(IPT)是细胞分裂素合成中的关键限速酶,在植物生长中起着至关重要的作用,发展,和对非生物胁迫的抗性。
结果:与野生型相比,转基因爬行弯草表现出较慢的生长速度,提高耐旱性,和改善的耐荫性归因于延迟的叶片衰老。此外,转基因植物显示抗氧化酶水平显着增加,叶绿素含量,和可溶性糖。重要的是,这项研究发现,MtIPT基因的过表达不仅显着提高了细胞分裂素和生长素的含量,而且还影响了油菜素类固醇的水平。RNA-seq分析显示,转基因和野生型植物的差异表达基因(DEGs)与植物激素信号转导密切相关,类固醇生物合成,光合作用,类黄酮生物合成,类胡萝卜素生物合成,花青素生物合成,氧化还原过程,细胞分裂素代谢,和蜡生物合成。和许多与增长相关的DEG,发展,并确定了胁迫耐受性,包括细胞分裂素信号转导基因(CRE1,B-ARR),抗氧化酶相关基因(APX2,PEX11,PER1),光合作用相关基因(ATPF1A,PSBQ,PETF),类黄酮合成基因(F3H,C12RT1,DFR),蜡合成基因(MAH1),衰老相关基因(SAG20),在其他人中。
结论:这些发现表明,MtIPT基因作为植物生长和发育的负调节因子,同时在植物对非生物胁迫的反应中也起着至关重要的作用。
BACKGROUND: Isopentenyltransferases (IPT) serve as crucial rate-limiting enzyme in cytokinin synthesis, playing a vital role in plant growth, development, and resistance to abiotic stress.
RESULTS: Compared to the wild type, transgenic creeping bentgrass exhibited a slower growth rate, heightened drought tolerance, and improved shade tolerance attributed to delayed leaf senescence. Additionally, transgenic plants showed significant increases in antioxidant enzyme levels, chlorophyll content, and soluble sugars. Importantly, this study uncovered that overexpression of the MtIPT gene not only significantly enhanced cytokinin and auxin content but also influenced brassinosteroid level. RNA-seq analysis revealed that differentially expressed genes (DEGs) between transgenic and wild type plants were closely associated with plant hormone signal transduction, steroid biosynthesis, photosynthesis, flavonoid biosynthesis, carotenoid biosynthesis, anthocyanin biosynthesis, oxidation-reduction process, cytokinin metabolism, and wax biosynthesis. And numerous DEGs related to growth, development, and stress tolerance were identified, including cytokinin signal transduction genes (CRE1, B-ARR), antioxidase-related genes (APX2, PEX11, PER1), Photosynthesis-related genes (ATPF1A, PSBQ, PETF), flavonoid synthesis genes (F3H, C12RT1, DFR), wax synthesis gene (MAH1), senescence-associated gene (SAG20), among others.
CONCLUSIONS: These findings suggest that the MtIPT gene acts as a negative regulator of plant growth and development, while also playing a crucial role in the plant\'s response to abiotic stress.