PDF(Pubmed)
Abstract：
Halogenated phenazine meroterpenoids are a structurally unusual family of marine actinobacterial natural products that exhibit antibiotic, antibiofilm, and cytotoxic bioactivities. Despite a lack of established phenazine halogenation biochemistry, genomic analysis of Streptomyces sp. CNZ-289, a prolific lavanducyanin and C2-halogenated derivative producer, suggested the involvement of vanadium-dependent haloperoxidases. We subsequently discovered lavanducyanin halogenase (LvcH), characterized it in vitro as a regioselective vanadium-dependent chloroperoxidase, and applied it in late-stage chemoenzymatic synthesis.
摘要：
卤化吩嗪类汞是一种结构上不寻常的海洋放线菌天然产物家族，表现出抗生素，抗生物膜,和细胞毒性生物活性。尽管缺乏既定的吩嗪卤化生物化学，链霉菌属的基因组分析。CNZ-289，多产的lavanducyanin和C2-卤化衍生物生产商，提示钒依赖性卤代过氧化物酶的参与。我们随后发现了lavanducyanin卤化酶（LvcH），在体外将其表征为区域选择性钒依赖性氯过氧化物酶，并将其应用于后期化学酶法合成。
