{Reference Type}: Journal Article
{Title}: Regioselective Halogenation of Lavanducyanin by a Site-Selective Vanadium-Dependent Chloroperoxidase.
{Author}: Baumgartner JT;McKinnie SMK;
{Journal}: Org Lett
{Volume}: 26
{Issue}: 27
{Year}: 2024 Jul 12
{Factor}: 6.072
{DOI}: 10.1021/acs.orglett.4c01869
{Abstract}: Halogenated phenazine meroterpenoids are a structurally unusual family of marine actinobacterial natural products that exhibit antibiotic, antibiofilm, and cytotoxic bioactivities. Despite a lack of established phenazine halogenation biochemistry, genomic analysis of Streptomyces sp. CNZ-289, a prolific lavanducyanin and C2-halogenated derivative producer, suggested the involvement of vanadium-dependent haloperoxidases. We subsequently discovered lavanducyanin halogenase (LvcH), characterized it in vitro as a regioselective vanadium-dependent chloroperoxidase, and applied it in late-stage chemoenzymatic synthesis.