PDF(Pubmed)
Abstract：
The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected to a wide range of RNA modifications. In this study, we focused on the chikungunya virus (CHIKV) as a model (+) ssRNA virus to study the landscape of viral RNA modification in infected human cells. Among the 32 distinct RNA modifications analysed by mass spectrometry, inosine was found enriched in the genomic CHIKV RNA. However, orthogonal validation by Illumina RNA-seq analyses did not identify any inosine modification along the CHIKV RNA genome. Moreover, CHIKV infection did not alter the expression of ADAR1 isoforms, the enzymes that catalyse the adenosine to inosine conversion. Together, this study highlights the importance of a multidisciplinary approach to assess the presence of RNA modifications in viral RNA genomes.
摘要：
认为正(+)单链RNA(ssRNA)病毒的基因组经受广泛的RNA修饰。在这项研究中,我们专注于基孔肯雅病毒（CHIKV）作为模型（）ssRNA病毒，以研究受感染的人类细胞中病毒RNA修饰的景观。在通过质谱分析的32种不同的RNA修饰中，发现肌苷富集在基因组CHIKVRNA中。然而,通过IlluminaRNA-seq分析进行的正交验证未发现CHIKVRNA基因组上有任何肌苷修饰.此外，CHIKV感染没有改变ADAR1亚型的表达，催化腺苷向肌苷转化的酶。一起,这项研究强调了多学科方法评估病毒RNA基因组中RNA修饰的重要性.
