PDF(Pubmed)
Abstract：
Filamentous bacteriophages belonging to the order Tubulavirales, family Inoviridae, significantly affect the properties of Gram-negative bacteria, but filamentous phages of many important pathogens have not been described so far. The aim of this study was to examine A. baumannii filamentous phages for the first time and to determine their effect on bacterial virulence. The filamentous phages were detected in 15.3% of A. baumannii strains as individual prophages in the genome or as tandem repeats, and a slightly higher percentage was detected in the culture collection (23.8%). The phylogenetic analyses revealed 12 new genera within the Inoviridae family. Bacteriophages that were selected and isolated showed structural and genomic characteristics of the family and were unable to form plaques. Upon host infection, these phages did not significantly affect bacterial twitching motility and capsule production but significantly affected growth kinetics, reduced biofilm formation, and increased antibiotic sensitivity. One of the possible mechanisms of reduced resistance to antibiotics is the observed decreased expression of efflux pumps after infection with filamentous phages.
摘要：
丝状噬菌体属于Tubulavirales，非病毒科,显著影响革兰氏阴性菌的特性，但是到目前为止还没有描述许多重要病原体的丝状噬菌体。这项研究的目的是首次检查鲍曼不动杆菌丝状噬菌体，并确定其对细菌毒力的影响。在15.3％的鲍曼不动杆菌菌株中检测到丝状噬菌体，作为基因组中的单个噬菌体或串联重复序列，在培养物中检测到的百分比略高（23.8％）。系统发育分析揭示了Inoviridae家族中的12个新属。选择和分离的噬菌体显示了该家族的结构和基因组特征，无法形成斑块。在宿主感染时，这些噬菌体没有显着影响细菌抽搐运动和胶囊生产，但显着影响生长动力学，减少生物膜形成，增加抗生素敏感性。对抗生素耐药性降低的可能机制之一是在丝状噬菌体感染后观察到外排泵的表达降低。
