{Reference Type}: Journal Article
{Title}: First Characterization of Acinetobacter baumannii-Specific Filamentous Phages.
{Author}: Narancic J;Gavric D;Kostanjsek R;Knezevic P;
{Journal}: Viruses
{Volume}: 16
{Issue}: 6
{Year}: 2024 May 27
{Factor}: 5.818
{DOI}: 10.3390/v16060857
{Abstract}: Filamentous bacteriophages belonging to the order Tubulavirales, family Inoviridae, significantly affect the properties of Gram-negative bacteria, but filamentous phages of many important pathogens have not been described so far. The aim of this study was to examine A. baumannii filamentous phages for the first time and to determine their effect on bacterial virulence. The filamentous phages were detected in 15.3% of A. baumannii strains as individual prophages in the genome or as tandem repeats, and a slightly higher percentage was detected in the culture collection (23.8%). The phylogenetic analyses revealed 12 new genera within the Inoviridae family. Bacteriophages that were selected and isolated showed structural and genomic characteristics of the family and were unable to form plaques. Upon host infection, these phages did not significantly affect bacterial twitching motility and capsule production but significantly affected growth kinetics, reduced biofilm formation, and increased antibiotic sensitivity. One of the possible mechanisms of reduced resistance to antibiotics is the observed decreased expression of efflux pumps after infection with filamentous phages.