PDF(Pubmed)
Abstract：
Corn peptide (CP) is a short, naturally occurring, and physiologically active peptide generated from corn-protease-catalyzed hydrolysis. CP plays a role in preventing obesity-related disorders, but its impact on reducing inflammation is unknown. Hence, this study examined the possible protective effects of corn peptide powder (CPP) against the harmful effects of lipopolysaccharide (LPS), with a particular emphasis on reducing oxidative damage and inflammation in adipocytes. Hence, mature 3T3-L1 adipocytes underwent exposure to 10 ng/mL LPS, with or without CPP (10 and 20 μg/mL). LPS stimulation increased reactive oxygen species and superoxide anion generation. However, this effect was reduced in a dose-dependent manner by pretreatment with CPP. CPP treatment elevated the mRNA expressions of the antioxidant enzymes manganese superoxide dismutase (mnSOD) and glutathione peroxidase 1 (Gpx1) while reducing the mRNA expressions of the cytosolic reactive oxygen species indicators p40 and p67 (NADPH oxidase 2). In addition, CPP inhibited the monocyte chemoattractant protein-1, tumor necrosis factor-alpha, Toll-like receptor 4, and nuclear factor kappa B mRNA expressions induced by LPS. These findings demonstrate that CPP may ameliorate adipocyte dysfunction by suppressing oxidative damage and inflammatory responses through a new mechanism known as Toll-like receptor 4/nuclear factor kappa B-mediated signaling.
摘要：
玉米肽（CP）是一种短，自然发生,和从玉米蛋白酶催化水解产生的生理活性肽。CP在预防肥胖相关疾病中起作用，但它对减少炎症的影响尚不清楚。因此，这项研究检查了玉米肽粉（CPP）对脂多糖（LPS）有害作用的可能的保护作用，特别强调减少脂肪细胞的氧化损伤和炎症。因此，成熟的3T3-L1脂肪细胞暴露于10ng/mLLPS，有或没有CPP（10和20μg/mL）。LPS刺激增加了活性氧和超氧阴离子的产生。然而,通过CPP预处理,这种效应以剂量依赖性方式降低.CPP处理提高了抗氧化酶锰超氧化物歧化酶（mnSOD）和谷胱甘肽过氧化物酶1（Gpx1）的mRNA表达，同时降低了胞浆活性氧指标p40和p67（NADPH氧化酶2）的mRNA表达。此外,CPP抑制单核细胞趋化蛋白-1，肿瘤坏死因子-α，LPS诱导Toll样受体4和核因子κBmRNA表达。这些发现表明，CPP可能通过一种称为Toll样受体4/核因子κB介导的信号传导的新机制来抑制氧化损伤和炎症反应，从而改善脂肪细胞功能障碍。
