PDF(Pubmed)
Abstract：
Secondary metabolites, bioactive compounds produced by living organisms, can unveil symbiotic relationships in nature. In this study, soilborne entomopathogenic nematodes associated with symbiotic bacteria (Xenorhabdus stockiae and Photorhabdus luminescens) were extracted from solvent supernatant containing secondary metabolites, demonstrating significant inhibitory effects against E. coli, S. aureus, B. subtilus, P. mirabilis, E. faecalis, and P. stutzeri. The characterization of these secondary metabolites by Fourier transforms infrared spectroscopy revealed amine groups of proteins, hydroxyl and carboxyl groups of polyphenols, hydroxyl groups of polysaccharides, and carboxyl groups of organic acids. Furthermore, the obtained crude extracts were analyzed by high-performance liquid chromatography for the basic identification of potential bioactive peptides. Gas chromatography-mass spectrometry analysis of ethyl acetate extracts from Xenorhabdus stockiae identified major compounds including nonanoic acid derivatives, proline, paromycin, octodecanal derivatives, trioxa-5-aza-1-silabicyclo, 4-octadecenal, methyl ester, oleic acid, and 1,2-benzenedicarboxylicacid. Additional extraction from Photorhabdus luminescens yielded functional compounds such as indole-3-acetic acid, phthalic acid, 1-tetradecanol, nemorosonol, 1-eicosanol, and unsaturated fatty acids. These findings support the potential development of novel natural antimicrobial agents for future pathogen suppression.
摘要：
次级代谢产物,活生物体产生的生物活性化合物，可以揭示自然界中的共生关系。在这项研究中,从含有次生代谢产物的溶剂上清液中提取与共生细菌（Xenorhabdusstockiae和Photorhabdusluminescens）相关的土传昆虫病原线虫，证明了对大肠杆菌的显著抑制作用，金黄色葡萄球菌，B.subtilus,P.奇迹,E.粪便，还有P.Stutzeri.通过傅立叶变换红外光谱对这些次生代谢物的表征揭示了蛋白质的胺基，多酚的羟基和羧基，多糖的羟基，和有机酸的羧基。此外，通过高效液相色谱法分析获得的粗提物，以基本鉴定潜在的生物活性肽。气相色谱-质谱分析来自Xenorhabdusstoriae的乙酸乙酯提取物确定了主要化合物，包括壬酸衍生物，脯氨酸,巴霉素,八癸醛衍生物，三氧杂-5-氮杂-1-硅双环，4-十八进制,甲酯，油酸,和1,2-苯二羧酸。从发光光纹素中进行额外的提取，产生了功能化合物，例如吲哚-3-乙酸，邻苯二甲酸,1-十四醇,奈莫索诺,1-二十烷醇,和不饱和脂肪酸。这些发现支持用于未来病原体抑制的新型天然抗微生物剂的潜在开发。
