PDF(Pubmed)
Abstract：
Bletilla striata is the dried tuber of B. striata (Thund.) Reichb.f., which has antibacterial, anti-inflammatory, anti-tumor, antioxidant and wound healing effects. Traditionally, it has been used for hemostasis therapy, as well as to treat sores, swelling and chapped skin. In this study, we used the ultraviolet (UV) absorbance rate of B. striata extracts as the index, and the extraction was varied with respect to the solid-liquid ratio, ethanol concentration, ultrasonic time and temperature in order to optimize the extraction process for its sunscreen components. The main compounds in the sunscreen ingredients of Baiji (B. striata) were analyzed using ultra-high-performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry. The sunscreen properties were subsequently evaluated in vitro using the 3M tape method. The results show that the optimal extraction conditions for the sunscreen components of B. striata were a solid-liquid ratio of 1:40 (g/mL), an ethanol concentration of 50%, an ultrasonic time of 50 min and a temperature of 60 °C. A power of 100 W and an ultrasonic frequency of 40 Hz were used throughout the experiments. Under these optimized conditions, the UV absorption rate of the isolated sunscreen components in the UVB region reached 84.38%, and the RSD was 0.11%. Eighteen compounds were identified, including eleven 2-isobutyl malic acid glucose oxybenzyl esters, four phenanthrenes, two bibenzyl and one α-isobutylmalic acid. An evaluation of the sunscreen properties showed that the average UVB absorption values for the sunscreen samples from different batches of B. striata ranged from 0.727 to 1.201. The sunscreen ingredients of the extracts from B. striata had a good UV absorption capacity in the UVB area, and they were effective in their sunscreen effects under medium-intensity sunlight. Therefore, this study will be an experimental reference for the extraction of sunscreen ingredients from the B. striata plant, and it provides evidence for the future development of B. striata as a candidate cosmetic raw material with UVB protection properties.
摘要：
白菜是B.striata（Thund。)Reichb.f.，具有抗菌性，抗炎,抗肿瘤，抗氧化和伤口愈合效果。传统上,它已被用于止血治疗，以及治疗疮，皮肤肿胀和干裂。在这项研究中,我们以纹状体芽孢杆菌提取物的紫外线（UV）吸收率为指标，并且提取根据固液比而变化，乙醇浓度，超声时间和温度,以优化其防晒成分的提取工艺。百济防晒成分中的主要化合物（B.纹状体）使用超高效液相色谱结合四极杆飞行时间串联质谱进行分析。随后使用3M胶带方法在体外评价防晒性能。结果表明,纹状体防晒成分的最佳提取条件为料液比1:40(g/mL),乙醇浓度为50%,超声时间为50分钟，温度为60°C。在整个实验中使用100W的功率和40Hz的超声频率。在这些优化条件下，分离的防晒成分在UVB区域的紫外线吸收率达到84.38%,RSD为0.11%。鉴定出18种化合物,包括11种2-异丁基苹果酸葡萄糖氧苄酯，四种菲，两个联苄和一个α-异丁基苹果酸。对防晒剂性质的评估显示,来自不同批次的纹状体芽孢杆菌的防晒剂样品的平均UVB吸收值在0.727至1.201的范围内。纹状体提取物的防晒成分在UVB区域具有良好的紫外线吸收能力，它们在中等强度的阳光下具有有效的防晒效果。因此,这项研究将为从B.striata植物中提取防晒成分提供实验参考，它为B.striata作为具有UVB保护性能的候选化妆品原料的未来发展提供了证据。
