输卵管异常,一种富含抗肿瘤活性的中药植物,需要有效的提取方法。在本文中,首先建立了一种高效液相色谱检测方法,使用甲醇-水溶剂系统进行梯度洗脱。发现在T.alomalum的叶子中Nutolides的含量最高,其次是茎和果实,几乎没有根。在实际采摘过程中,收集的叶子数量相对较少,而茎的数量最高。因此,采用Box-Behnken响应面法,优化了超声波辅助提取T.alomalum茎中胡椒素的工艺。确定了最佳提取条件:液固比为20:1,提取溶剂为70%乙醇,超声波功率为250W,超声时间为40分钟,超声波温度为50℃。在这些条件下,单肽A(Te-A)和单肽A(Ta-A)的平均产量可达到2.87±0.12mg/g和1.18±0.05mg/g,分别。我们进一步比较了使用超声波和传统提取方法从T。异常的不同部位提取两种内酯的提取率。超声波提取率显着提高,树叶产量最高,其次是茎和水果。结果表明,超声波优化可以提高提取率,减少时间,更低的成本,提高质量,并提高产量。因此,采用优化的超声波辅助提取工艺对T.alomalum的地上部分进行提取并分离。优化后,提取物经历了几次色谱分离,以分离出八个先前未描述的与anolides(1-8)和两个人工与anolides(9-10),除了15个已知的化合物(11-25)。它们的结构是通过广泛的光谱数据分析建立的。评估了化合物对多种癌细胞系的抗增殖作用,包括人肝癌细胞(HepG2,Hep3B,和MHCC97-H),人肺癌细胞(A549),人纤维肉瘤癌细胞(HT1080),人慢性粒细胞白血病细胞(K562),和人乳腺癌细胞(MDA-MB-231和MCF7)。化合物1-3、5、7、11、13、15-16和22显示出显著的活性,IC50值为5.14-19.87μM。以上结果表明,超声波辅助提取技术可以更有效地从T。从而提高了T.异常资源的利用率。
Tubocapsicum anomalum, a Chinese medicinal plant rich in anti-tumor withanolides, requires efficient extraction methods. In this paper, an HPLC method was first established for the detection of withanolides, and gradient elution was carried out using a methanol-water solvent system. It was found that the content of withanolides was the highest in the leaves of T. anomalum, followed by the stems and fruits, and almost none in the roots. During the actual picking process, the quantity of leaves collected was relatively small, while the number of stems was the highest. Therefore, the Box-Behnken response surface method was used to optimize the ultrasonic-assisted extraction process of withanolides from the stems of T. anomalum. The optimal extraction conditions were determined as follows: the liquid-solid ratio was 20:1, the extraction solvent was 70 % ethanol, the ultrasonic power was 250 W, the ultrasonic time was 40 min, and the ultrasonic temperature was 50 °C. Under these conditions, the average yields of tubocapsenolide A (Te-A) and tubocapsanolide A (Ta-A) can reach 2.87 ± 0.12 mg/g and 1.18 ± 0.05 mg/g, respectively. We further compared extraction rates of two withanolides from different parts of T. anomalum using ultrasonic and traditional extraction methods. Ultrasonic extraction significantly increased rates, with the highest yields from leaves, followed by stems and fruits. The results show that ultrasonic optimization can improve extraction rate, reduce time, lower costs, enhance quality, and increase yield. Therefore, the optimized ultrasonic-assisted extraction process was adopted to extract the aerial parts of T. anomalum and separate the components. After optimization, the extract underwent several chromatographic separations to isolate eight previously undescribed withanolides (1-8) and two artificial withanolides (9-10), in addition to fifteen known compounds (11-25). Their structures were established through extensive spectroscopic data analysis. The compounds were evaluated for their antiproliferative effects against multiple cancer cell lines, including human hepatocellular carcinoma cells (HepG2, Hep3B, and MHCC97-H), human lung cancer cells (A549), human fibro-sarcoma cancer cells (HT1080), human chronic myeloid leukemia cells (K562), and human breast cancer cells (MDA-MB-231 and MCF7). Compounds 1-3, 5, 7, 11, 13, 15-16, and 22 displayed significant activity with IC50 values of 5.14-19.87 μM. The above results indicate that ultrasonic-assisted extraction technology can be used to obtain new withanolides more efficiently from T. anomalum, thereby enhancing the utilization rate of T. anomalum resources.