PDF(Pubmed)
Abstract：
The content of chemical constituents in Eugenia uniflora leaf extracts correlates positively with biological activities. The experimental objective was to carry out the phytochemical screening and purification of the major polyphenols from the leaves of E. uniflora. In addition, the anti-Candida activity of the hydroalcoholic extract, fraction, subfractions and polyphenols purified were evaluated. After partitioning of the extract with ethyl acetate, the fractions were chromatographed on Sephadex® LH-20 gel followed by RP-flash chromatography and monitored by TLC and RP-HPLC. The samples were characterized by mass spectrometry (LC-ESI-QTOF-MS2) and subjected to the microdilution method in 96-well plates against strains of C. albicans, C. auris, and C. glabrata. Myricitrin (93.89%; w/w; m/z 463.0876), gallic acid (99.9%; w/w; m/z 169.0142), and ellagic acid (94.2%; w/w; m/z 300.9988) were recovered. The polyphenolic fraction (62.67% (w/w) myricitrin) and the ellagic fraction (67.86% (w/w) ellagic acid) showed the best antifungal performance (MIC between 62.50 and 500 μg/mL), suggesting an association between the majority constituents and the antifungal response of E. uniflora derivatives. However, there is a clear dependence on the presence of the complex chemical mixture. In conclusion, chromatographic strategies were effectively employed to recover the major polyphenols from the leaves of the species.
摘要：
杜松子花叶提取物中化学成分的含量与生物活性呈正相关。本实验目的是进行植物化学筛选，并从E.uniflora的叶片中纯化主要的多酚。此外,水醇提取物的抗念珠菌活性，分数，亚组分和多酚纯化进行了评估。用乙酸乙酯分配提取物后，将级分在Sephadex®LH-20凝胶上进行色谱分离,然后进行RP-快速色谱,并通过TLC和RP-HPLC监测。样品通过质谱（LC-ESI-QTOF-MS2）进行表征，并在96孔板中对白色念珠菌菌株进行微量稀释法，C.auris,和C.glabrata.杨梅苷（93.89%；w/w；m/z463.0876），没食子酸(99.9%;w/w;m/z169.0142),回收鞣花酸(94.2%;w/w;m/z300.9988)。多酚部分（62.67％（w/w）杨梅苷）和鞣层部分（67.86％（w/w）鞣花酸）显示出最佳的抗真菌性能（MIC在62.50至500μg/mL之间），表明大多数成分与E.uniflora衍生物的抗真菌反应之间存在关联。然而,对复杂化学混合物的存在有明显的依赖性。总之,色谱策略被有效地用于从该物种的叶子中回收主要的多酚。
