PDF(Pubmed)
Abstract：
University dormitories represent densely populated environments, and washing machines are potential sites for the spread of bacteria and microbes. However, the extent of antibiotic resistance gene (ARG) variation in washing machines within university dormitories and their potential health risks are largely unknown. To disclose the occurrence of ARGs and antibiotic-resistant bacteria from university dormitories, we collected samples from washing machines in 10 dormitories and used metagenomic sequencing technology to determine microbial and ARG abundance. Our results showed abundant microbial diversity, with Proteobacteria being the dominant microorganism that harbors many ARGs. The majority of the existing ARGs were associated with antibiotic target alteration and efflux, conferring multidrug resistance. We identified tnpA and IS91 as the most abundant mobile genetic elements (MGEs) in washing machines and found that Micavibrio aeruginosavorus, Aquincola tertiaricarbonis, and Mycolicibacterium iranicum had high levels of ARGs. Our study highlights the potential transmission of pathogens from washing machines to humans and the surrounding environment. Pollution in washing machines poses a severe threat to public health and demands attention. Therefore, it is crucial to explore effective methods for reducing the reproduction of multidrug resistance.
摘要：
大学宿舍代表人口稠密的环境，洗衣机是细菌和微生物传播的潜在场所。然而,大学宿舍洗衣机中抗生素耐药基因(ARG)变异的程度及其潜在的健康风险在很大程度上是未知的.披露来自大学宿舍的ARGs和抗生素抗性细菌的发生，我们从10个宿舍的洗衣机中收集样本，并使用宏基因组测序技术确定微生物和ARG丰度。我们的结果显示了丰富的微生物多样性，变形杆菌是含有许多ARG的主要微生物。大多数现有的ARGs与抗生素靶标改变和外排有关，赋予多药耐药性。我们确定tnpA和IS91是洗衣机中最丰富的可移动遗传元件（MGEs），并发现铜绿微菌，阿奎莫拉tertiariconbonis，和酵母菌有高水平的ARGs。我们的研究强调了病原体从洗衣机向人类和周围环境的潜在传播。洗衣机的污染对公众健康构成严重威胁，需要引起注意。因此,探索减少多药耐药繁殖的有效方法至关重要。
