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Abstract：
The present study reports on the preparation of a cellulose fiber (CF) composite from D. lutescens, combined with copper oxide nanoparticles (DL@CF/CuO), to prolong the shelf life of tomatoes after harvest. The isolated cellulose fiber material was comprehensively characterized using XRD, FTIR, and FE-SEM analyses. The DLCF and DL@CF/CuO nanoparticles exhibited crystalline cellulose, as indicated by the XRD investigation. Both DLCF and DL@CF/CuO showed O-H and C-H FTIR spectra with identifiable vibrational peaks. The FE-SEM images depicted the dispersion of DL@CF/CuO-based fibers in a cellulose fiber matrix containing CuO nanoparticles. A 0.3% (wt/wt), a solution of DL@CF/CuO was coated onto the surface of early ripening tomato fruits. After a 25-day storage period at 25-29 °C and 85% RH, the results showed a significant extension in the shelf life of the tomato fruits, in line with changes in physiological properties and fruit quality. The extension of shelf life in tomato fruit epidermis treated with DL@CF/CuO was confirmed through FE-SEM analysis. L929 fibroblast cells were treated with the developed DL@CF/CuO nanocomposite, and no signs of toxicity were detected up to 75 µg/mL. Additionally, the DL@CF/CuO nanocomposite exhibited significant antifungal activity against Aspergillus flavus. In conclusion, this study provides novel insights for sustainable food security and waste control in the agricultural and food industries.
摘要：
本研究报道了从D.lutescens制备纤维素纤维（CF）复合材料，结合氧化铜纳米颗粒（DL@CF/CuO），延长西红柿收获后的保质期。利用XRD对分离的纤维素纤维材料进行了全面表征，FTIR,和FE-SEM分析。DLCF和DL@CF/CuO纳米粒子表现出结晶纤维素，如XRD研究所示。DLCF和DL@CF/CuO均显示具有可识别的振动峰的O-H和C-HFTIR光谱。FE-SEM图像描绘了DL@CF/CuO基纤维在含有CuO纳米颗粒的纤维素纤维基质中的分散。0.3%(wt/wt)，将DL@CF/CuO溶液涂覆在早熟番茄果实的表面上。在25-29°C和85%RH下储存25天后，结果表明，番茄果实的保质期显著延长，符合生理特性和果实品质的变化。通过FE-SEM分析证实了用DL@CF/CuO处理的番茄果实表皮的保质期延长。用开发的DL@CF/CuO纳米复合材料处理L929成纤维细胞，并且在高达75µg/mL时没有检测到毒性迹象。此外,DL@CF/CuO纳米复合材料对黄曲霉具有显著的抗真菌活性。总之,这项研究为农业和食品行业的可持续粮食安全和废物控制提供了新的见解。
